Abstract
The complete mitochondrial genome sequences of Brassica species have provided insight into inter- and intraspecific variation of plant mitochondrial genomes. However, the size of mitochondrial genome sequenced for Brassica oleracea hitherto does not match to its physical mapping data. This fact led us to investigate B. oleracea mitochondrial genome in detail. Here we report novel B. oleracea mitochondrial genome, derived from var. capitata, a cabbage cultivar ‘‘Fujiwase’’. The genome was assembled into a 219,952-bp circular sequence that is comparable to the mitochondrial genomes of other Brassica species (ca. 220–232 kb). This genome contained 34 protein-coding genes, 3 rRNA genes and 17 tRNA genes. Due to absence of a large repeat (140 kb), the mitochondrial genome of ‘‘Fujiwase’’ is clearly smaller than the previously reported mitochondrial genome of B. oleracea accession ‘‘08C717’’ (360 kb). In both mitotypes, all genes were identical, except cox2-2, which was present only in the Fujiwase type. At least two rearrangement events via large and small repeat sequences have contributed to the structural differences between the two mitotypes. PCR-based marker analysis revealed that the Fujiwase type is predominant, whereas the 08C717 type coexists at low frequency in all B. oleracea cultivars examined. Intraspecific variations in the mitochondrial genome in B. oleracea may occur because of heteroplasmy, coexistence of different mitotypes within an individual, and substoichiometric shifting. Our data indicate that the Fujiwase-type genome should be used as the representative genome of B. oleracea.
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Acknowledgments
This study was supported in part by the Private University Strategic Research Foundation Support Program, Grants-in-Aid for Scientific Research, Scientific Research (B) (No. 22380008), and the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN), Japan.
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Communicated by S. Hohmann.
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294_2014_433_MOESM1_ESM.pdf
Validation of contig linkage by PCR analysis. (A) Map of linkages between contigs. (B) PCR analysis to confirm the linkages. The primer information used for this PCR analysis is described in Supplementary Table 1 (PDF 221 kb)
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Tanaka, Y., Tsuda, M., Yasumoto, K. et al. The complete mitochondrial genome sequence of Brassica oleracea and analysis of coexisting mitotypes. Curr Genet 60, 277–284 (2014). https://doi.org/10.1007/s00294-014-0433-2
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DOI: https://doi.org/10.1007/s00294-014-0433-2