Research Article

Current Genetics

, Volume 59, Issue 4, pp 231-242

Open Access This content is freely available online to anyone, anywhere at any time.

The emergence of lipid droplets in yeast: current status and experimental approaches

  • Maja RadulovicAffiliated withInstitute of Molecular Biosciences, University of Graz
  • , Oskar KnittelfelderAffiliated withInstitute of Molecular Biosciences, University of Graz
  • , Alvaro Cristobal-SarramianAffiliated withInstitute of Molecular Biosciences, University of Graz
  • , Dagmar KolbAffiliated withInstitute of Cell Biology, Histology and Embryology and Center for Medical Research, Medical University of Graz
  • , Heimo WolinskiAffiliated withInstitute of Molecular Biosciences, University of Graz
  • , Sepp D. KohlweinAffiliated withInstitute of Molecular Biosciences, University of Graz Email author 

Abstract

The ‘discovery’ of lipid droplets as a metabolically highly active subcellular organelle has sparked great scientific interest in its research in recent years. The previous view of a rather inert storage pool of neutral lipids—triacylglycerol and sterols or steryl esters—has markedly changed. Driven by the endemic dimensions of lipid-associated disorders on the one hand, and the promising biotechnological application to generate oils (‘biodiesel’) from single-celled organisms on the other, multiple model organisms are exploited in basic and applied research to develop a better understanding of biogenesis and metabolism of this organelle. This article summarizes the current status of LD research in yeast and experimental approaches to obtain insight into the regulatory and structural components driving lipid droplet formation and their physiological and pathophysiological roles in lipid homeostasis.

Keywords

Neutral lipid homeostasis Triacylglycerol Fluorescence microscopy CARS microscopy Electron microscopy Electron tomography Lipidomics