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The spectrum of spontaneous mutations caused by deficiency in proteasome maturase Ump1 in Saccharomyces cerevisiae

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Abstract

Ump1 is responsible for maturation of the catalytic core of the 26S proteasome. Dysfunction of Ump1 causes an increase in the frequency of spontaneous mutations in Saccharomyces cerevisiae. In this study we analyze the spectrum of mutations occurring spontaneously in yeast deficient in Ump1 by use of the SUP4-o system. Single base substitutions predominate among the mutations analyzed (73 of the 91 alterations examined). Two major classes are GC to TA transversions and GC to AT transitions (∼50 and ∼30% of base substitutions, respectively). Besides base substitutions, almost all the major types of sequence alterations are represented. The specificity and distribution of mutations occurring in the ump1 strain are unique compared to the spectra previously established for other yeast mutators. However, the profile of mutations arising in this strain is similar to that observed in wild type. The same similarity has previously been reported for yeast deficient in Mms2, a protein involved in Rad6-dependent postreplication DNA repair (PRR). The specificity of the mutator effect caused by ump1 is discussed in light of the proposed role of the proteasome activity in the regulation of the PRR mechanisms.

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Acknowledgments

We thank Dr. Bernard Kunz for providing the SUP4-o system and the experimental protocol for determination of the spectrum of mutagenesis. This work was supported by grant N303 040 31/1306 from the Polish Ministry of Education and Science.

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Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawinskiego 5A, 02-106, Warsaw, Poland

    Justyna McIntyre, Hanna Baranowska, Adrianna Skoneczna, Agnieszka Halas & Ewa Sledziewska-Gojska

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  1. Justyna McIntyre
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  2. Hanna Baranowska
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  3. Adrianna Skoneczna
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  4. Agnieszka Halas
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  5. Ewa Sledziewska-Gojska
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Corresponding author

Correspondence to Ewa Sledziewska-Gojska.

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Communicated by M.P. Longhese.

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McIntyre, J., Baranowska, H., Skoneczna, A. et al. The spectrum of spontaneous mutations caused by deficiency in proteasome maturase Ump1 in Saccharomyces cerevisiae . Curr Genet 52, 221–228 (2007). https://doi.org/10.1007/s00294-007-0156-8

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  • DOI: https://doi.org/10.1007/s00294-007-0156-8

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