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Sulfathiazole grafted PEG-PLL as pH-sensitive shielding system for cationic gene delivery

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Abstract

Polycationic gene carriers, mPEG-b-PLL-g-succinyl sulfathiazole (denoted as PPS), were synthesized and characterized as a shielding system for cationic gene delivery. DNA/PEI/PPS ternary complexes were prepared by shielding PPS to DNA/PEI polyplex solution. PPS can shield the surface charge of DNA/PEI polyplexes from positive (27.7 mV) to negative (−24.6 mV), and the acidic condition should trigger the disassembly of ternary complex. The gene transfection efficiency of DNA/PEI/PPS was lower than DNA/PEI at pH 7.4, but at pH 6.0, it recovered to the same level as DNA/PEI. CLSM results showed the same trend as transfection at different pH. The suitable pH-triggered shielding/deshielding ability estimated that PPS should be a potential shielding system to be used in in vivo gene delivery.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (51203132, 51303173, 21474104 and 51403205), Natural Science Foundation of Guangdong Province, China (S2012040008070) and Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (2012LYM_0093).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Lingnan Normal University, Zhanjiang, 524048, China

    Jialiang Xia, Bing Han, Hongyan Yang & Zongcai Feng

  2. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, 130022, China

    Jie Chen, Lin Lin, Zhaopei Guo & Huayu Tian

  3. Research Center of Chemistry and Materials, Lingnan Normal University, Development Center for New Materials Engineering and Technology in Universities of Guangdong, Zhanjiang, China

    Jialiang Xia & Zongcai Feng

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  1. Jialiang Xia
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  2. Jie Chen
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  3. Lin Lin
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  4. Zhaopei Guo
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  5. Bing Han
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  6. Hongyan Yang
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  7. Zongcai Feng
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  8. Huayu Tian
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Correspondence to Jialiang Xia or Huayu Tian.

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Xia, J., Chen, J., Lin, L. et al. Sulfathiazole grafted PEG-PLL as pH-sensitive shielding system for cationic gene delivery. Polym. Bull. 73, 3503–3511 (2016). https://doi.org/10.1007/s00289-016-1688-1

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  • DOI: https://doi.org/10.1007/s00289-016-1688-1

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