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An improved method for preparing glutaraldehyde cross-linked chitosan–poly(vinyl alcohol) microparticles

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Abstract

The features of microparticles, as size, surface structure, and morphology, depend, mainly, on the methodology used for their preparation. Emulsion polymerization techniques are undoubtedly among the most widespread. However, the use of toxic, volatile organic solvents represents a major disadvantage, namely, because of environmental issues. In this study, we prepared glutaraldehyde cross-linked chitosan–poly(vinyl alcohol) microparticles by an improved water-in-oil emulsion technique using corn oil as organic phase. The application of this polymeric blend as microparticle is scarcely investigated. As resulting of the procedure here presented, spherical and smooth surface microparticles were obtained, with mean diameter of 16 μm. The cross-linking reaction between the aldehyde and the amino or the hydroxyl groups formed either an imine (Schiff’s base) or an acetal bond, respectively, as analyzed by infrared spectroscopy. The microparticles here described did not present cytotoxic potential. Accordingly, this study can find promising and successful application in biotechnology.

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Acknowledgments

The authors thank to Vítor Redondo (Institute Pedro Nunes, Coimbra, Portugal) for providing the optical microscopy facility and for the particle size analysis. Authors also thank Prof. Ana Cristina Santos (Faculty of Medicine, University of Coimbra, Portugal) for conducting MTT assay.

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  1. Elisa Campos

    Present address: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, 2780-157, Oeiras, Portugal

Authors and Affiliations

  1. Chemical Process Engineering and Forest Products Research Centre (CIEPQPF), Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Rua Sílvio Lima, 3030-790, Coimbra, Portugal

    Elisa Campos, Patrícia Coimbra & M. H. Gil

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  1. Elisa Campos
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  2. Patrícia Coimbra
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  3. M. H. Gil
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Correspondence to Elisa Campos.

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Campos, E., Coimbra, P. & Gil, M.H. An improved method for preparing glutaraldehyde cross-linked chitosan–poly(vinyl alcohol) microparticles. Polym. Bull. 70, 549–561 (2013). https://doi.org/10.1007/s00289-012-0853-4

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  • DOI: https://doi.org/10.1007/s00289-012-0853-4

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