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Larkinella harenae sp. nov., Isolated from Korean Beach Soil

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Abstract

A Gram-negative, short rod-shaped, gliding motile, and pale pink-pigmented bacterial strain, designated 15J9-9T, was isolated from the soil of Iho Tewoo Beach, Jeju Island, Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J9-9T belonged to the family Cytophagaceae and was related to Larkinella bovis M2T2B15T (96.5% similarity), Larkinella arboricola Z0532T (95.3% similarity), and Larkinella insperata LMG 22510T (95.2% similarity). The DNA G+C content of strain 15J9-9T was 48 mol%. The detection of phosphatidylethanolamine, phosphatidylserine, two unknown aminophospholipids (APL1 and APL2), and two unknown polar lipids (L1 and L2) in polar lipid profile, menaquinone-7 as the predominant quinone, and a fatty acid profile of C16:1 ω5c, iso-C15:0, and iso-C17:0 3-OH as the major fatty acids supported the affiliation of strain 15J9-9T to the genus Larkinella. Based on its phenotypic properties and phylogenetic distinctiveness, strain 15J9-9T should be classified in the genus Larkinella as a representative of a novel species, for which the name Larkinella harenae sp. nov. is proposed. The type strain is 15J9-9T (=KCTC 42999T = JCM 31656T).

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Acknowledgements

This work was supported by the Human Resource Training Program for Regional Innovation and Creativity through the Ministry of Education and National Research Foundation (NRF) of Korea (NRF-2014H1C1A1066929) and by the Brain Pool Program of 2016 (Grant 162S-4-3-1727) through the Korean Federation of Science and Technology Societies (KOFST) funded by the Ministry of Science, ICT and Future Planning, Republic of Korea.

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Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Korea

    Su-Jin Park, Jae-Jin Lee, Seung-Yeol Lee, Leonid N. Ten & Hee-Young Jung

  2. Department of Environmental Engineering, Kyungpook National University, Daegu, 41566, Korea

    Dae Sung Lee

  3. Department of Bio and Environmental Technology, Seoul Women’s University, Seoul, 01797, Korea

    Myung Kyum Kim

  4. Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Korea

    Hee-Young Jung

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  1. Su-Jin Park
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Park, SJ., Lee, JJ., Lee, SY. et al. Larkinella harenae sp. nov., Isolated from Korean Beach Soil. Curr Microbiol 74, 798–802 (2017). https://doi.org/10.1007/s00284-017-1246-6

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