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Isolation, Characterization, and Bioinformatic Analyses of Lytic Salmonella Enteritidis Phages and Tests of Their Antibacterial Activity in Food

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Abstract

Salmonella Enteritidis remains a major threat for food safety. To take efforts to develop phage-based biocontrol for S. Enteritidis contamination in food, in this study, the phages against S. Enteritidis were isolated from sewage samples, characterized by host range assays, DNA restriction enzyme pattern analyses, and transmission electron microscope observations, and tested for antibacterial activity in food; some potent phages were further characterized by bioinformatic analyses. Results showed that based on the plaque quality and host range, seven lytic phages targeting S. Enteritidis were selected, considered as seven distinct phages through DNA physical maps, and classified as Myoviridae or Siphoviridae family by morphologic observations; the combined use of such seven strain phages as a “food additive” could succeed in controlling the artificial S. Enteritidis contamination in the different physical forms of food at a range of temperatures; by bioinformatic analyses, both selected phage BPS11Q3 and BPS15Q2 seemed to be newfound obligate lytic phage strains with no indications for any potentially harmful genes in their genomes. In conclusion, our results showed a potential of isolated phages as food additives for controlling S. Enteritidis contamination in some salmonellosis outbreak-associated food vehicles, and there could be minimized potential risk associated with using BPS11Q3 and BPS15Q2 in food.

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Acknowledgements

We are grateful to Dr. Hongduo Bao (Institute of Food Safety of JAAS), Dr. Baowei Yang (College of Food Science and Engineering of NAFU), and Dr. Zhongwei Liu (Guizhou University) for their cooperations. This work was supported by the National Natural Science Foundation of China (Grant Number 31302009) and Natural Science Foundation of Guizhou Education University (Grant Number 14YB010).

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Authors and Affiliations

  1. Key Laboratory of Guizhou Bioresource Development and Utilization, Guizhou Education University, Guiyang, 550018, Guizhou, China

    Han Han, Xiaoting Wei, Yi Wei, Xiufeng Zhang, Xuemin Li & Jinzhong Jiang

  2. Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Safety of Jiangsu Academy of Agricultural Science, Nanjing, 210014, Jiangsu, China

    Han Han & Ran Wang

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  1. Han Han
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  2. Xiaoting Wei
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  3. Yi Wei
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  4. Xiufeng Zhang
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  5. Xuemin Li
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  7. Ran Wang
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Correspondence to Ran Wang.

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Han, H., Wei, X., Wei, Y. et al. Isolation, Characterization, and Bioinformatic Analyses of Lytic Salmonella Enteritidis Phages and Tests of Their Antibacterial Activity in Food. Curr Microbiol 74, 175–183 (2017). https://doi.org/10.1007/s00284-016-1169-7

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  • DOI: https://doi.org/10.1007/s00284-016-1169-7

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