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Engineering Plasmid-Free Klebsiella Pneumoniae for Production of 3-Hydroxypropionic Acid

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Abstract

Although plasmid-dependent microbial breeding is predominant in manufacturing bio-based chemicals, it shows pitfall of genetic instability and thus hinders industrial production. Alternatively, chromosome engineering free from plasmid enables genetic stability and thus represents the new trend of microbial breeding. 3-Hydroxypropionic acid (3-HP) is an economically important platform compound as the versatile precursor of a suite of C3 compound, such as 1, 3-PDO. Klebsiella pneumoniae is regarded as a promising host strain due to both its dha regulon and exceptional glycerol fermentation ability. To produce 3-HP in K. pneumoniae, the IS1 region in chromosome was replaced with the AD DNA cassette containing aldH gene from E.coli through homologous recombination approach. The engineered recombinant converted glycerol into 3-HPA and then 3-HP when 40 g/L of initial glycerol was added. The novelties of this study comprise (i) the genetic stability of plasmid-free strains (ii) without using any inducer and antibiotics and thus more applicable than plasmid-based strains.

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Acknowledgement

This work is supported by National Natural Science Foundation of China (No. 21276014, 21476011).

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Authors and Affiliations

  1. Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union of Medical College, Beijing, People’s Republic of China

    Kang Wang

  2. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, People’s Republic of China

    Pingfang Tian

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  1. Kang Wang
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  2. Pingfang Tian
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Correspondence to Pingfang Tian.

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Wang, K., Tian, P. Engineering Plasmid-Free Klebsiella Pneumoniae for Production of 3-Hydroxypropionic Acid. Curr Microbiol 74, 55–58 (2017). https://doi.org/10.1007/s00284-016-1153-2

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  • DOI: https://doi.org/10.1007/s00284-016-1153-2

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