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Rhizobium albus sp. nov., Isolated from Lake Water in Xiamen, Fujian Province of China

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Abstract

A Gram-stain-negative, aerobic bacterial strain, designated Y21T, was isolated from surface lake water in Xiamen, Fujian Province of China. Growth was observed at temperatures from 4 to 37 °C, at salinities from 0 to 7.0 % and at pH from 6.0 to 10.0. Optimum growth was observed at 28 °C, at pH 7.0 and with 1.5–2.0 % (w/v) NaCl. The highest similarity of 16S rRNA gene sequence between strain Y21T and the other strains was 96.9 %. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the strain was a member of the genus Rhizobium, forming a distinct lineage with R. subbaraonis KCTC 23614T. The dominant fatty acids were summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c), C18:1 ω7c 11-methyl, which accounted for 78.1 %. The G+C content of the chromosomal DNA was 60.9 mol%. The predominant respiratory quinone was ubiquinone-10. The polar lipids of strain Y21T were found to consist of five unidentified phospholipids and three unidentified aminolipids. According to its morphology, physiology, fatty acid composition and 16S rRNA sequence data, strain Y21T should be regarded as a new species of the genus Rhizobium, for which Rhizobium albus sp. nov. is proposed (type strain Y21T = MCCC 1F01210T = KCTC 42252T).

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Abbreviations

MCCC:

Marine Culture Collection of China

KCTC:

Korean Collection For Type Cultures

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Acknowledgments

This work was supported by the NSFC (41576109, 31500095 and 31400318), the Doctoral Scientific Research Start-up Foundation of Henan Normal University (5101049170160), WEL Visiting Fellowship Program and the Key Scientific Research Programs of Henan Education Department (17A610002). We also thank Prof. I. J. Hodgkiss of The University of Hong Kong for comments and help with the English.

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Author notes

  1. Jinli Zhang

    Present address: Huang Chaoyang Building, Xiang’ an Campus, Xiamen University, Room 316, Xiamen, Fujian, China

Authors and Affiliations

  1. College of Life Sciences, Henan Normal University, Xinxiang, 453007, China

    Yi Li & Yanting Xu

  2. Key Lab of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen, 361005, China

    Xueqian Lei, Hong Zhu, Wei Zheng & Tianling Zheng

  3. Guangdong Institute of Microbiology, Guangzhou, 510070, China

    Meiying Xu

  4. College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China

    Jinli Zhang

  5. College of Environment and Life Science, Putian University, Putian, 351100, China

    Lijun Fu

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  1. Yi Li
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Correspondence to Jinli Zhang or Tianling Zheng.

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Conflict of Interest

We confirm that the manuscript has been read and approved by all named authors and that there are no other persons who satisfied the criteria for authorship but are not listed. We further confirm that the order of authors listed in the manuscript has been approved by all of us. There are no conflict of interest associated with this publication.

Additional information

Yi Li and Xueqian Lei have contributed equally to this work.

The GenBank [/EMBL/DDBJ] accession number for the 16S rRNA gene sequence of strain Y21T is KU245743.

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Li, Y., Lei, X., Xu, Y. et al. Rhizobium albus sp. nov., Isolated from Lake Water in Xiamen, Fujian Province of China. Curr Microbiol 74, 42–48 (2017). https://doi.org/10.1007/s00284-016-1149-y

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