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Characterization of a Rolling-Circle Replication Plasmid pM411 from Lactobacillus plantarum 1–3

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Abstract

A cryptic plasmid pM411 isolated from Lactobacillus plantarum 1–3 consisted of a 2303-bp circular molecule with a G + C content 32.96 %. Sequence analysis of pM411 revealed four putative open reading frames (ORFs). ORF1 shared 99 and 94 % similarities, respectively, with the Rep proteins of plasmids pLC2 and pYC2, which belong to the rolling-circle replication pMV158 family. A typical pMV158 family double-strand origin (dso) and a putative single-strand origin (sso) located upstream of the rep gene. Southern hybridization confirmed the presence of single-strand DNA (ssDNA) intermediates, suggesting that pM411 belongs to the RCR pMV158 family. Sequence homology analysis indicated that the sso belongs to the ssoW family. Furthermore, the relative copy number of pM411 was about 88 copies in each cell by real-time PCR.

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References

  1. Boer DR, Ruíz-Masó JA, López-Blanco JR, Blanco AG, Vives-Llàcer M, Chacón P, del Solar G (2009) Plasmid replication initiator RepB forms a hexamer reminiscent of ring helicases and has mobile nuclease domains. EMBO J 28(11):1666–1678

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Chen C, Ai L, Zhou F, Ren J, Sun K, Zhang H, Guo B (2012) Complete nucleotide sequence of plasmid pST-III from Lactobacillus plantarum ST-III. Plasmid 67(3):236–244

    Article  CAS  PubMed  Google Scholar 

  3. Cui Y, Hu T, Qu X, Zhang L, Ding Z, Dong A (2015) Plasmids from food lactic acid bacteria: diversity, similarity, and new developments. Int J Mol Sci 16(6):13172–13202

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Danielsen M (2002) Characterization of the tetracycline resistance plasmid pMD5057 from Lactobacillus plantarum 5057 reveals a composite structure. Plasmid 48(2):98–103

    Article  CAS  PubMed  Google Scholar 

  5. De Vries MC, Vaughan EE, Kleerebezem M, de Vos WM (2006) Lactobacillus plantarum—survival, functional and potential probiotic properties in the human intestinal tract. Int Dairy J 16(9):1018–1028

    Article  Google Scholar 

  6. Del Solar G, Giraldo R, Ruiz-Echevarría MJ, Espinosa M, Díaz-Orejas R (1998) Replication and control of circular bacterial plasmids. Microbiol Mol Biol Rev 62(2):434–464

    PubMed  PubMed Central  Google Scholar 

  7. Guglielmetti S, Mora D, Parini C (2007) Small rolling circle plasmids in Bacillus subtilis and related species: organization, distribution, and their possible role in host physiology. Plasmid 57(3):245–264

    Article  CAS  PubMed  Google Scholar 

  8. Heiss S, Grabherr R, Heinl S (2015) Characterization of the Lactobacillus plantarum plasmid pCD033 and generation of the plasmid free strain L. plantarum 3NSH. Plasmid 81:9–20

    Article  CAS  PubMed  Google Scholar 

  9. Jalilsood T, Baradaran A, Ling FH, Mustafa S, Yusof K, Rahim RA (2014) Characterization of pR18, a novel rolling-circle replication plasmid from Lactobacillus plantarum. Plasmid 73:1–9

    Article  CAS  PubMed  Google Scholar 

  10. Khan SA (1997) Rolling-circle replication of bacterial plasmids. Microbiol Mol Biol Rev 61(4):442–455

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Khan SA (2000) Plasmid rolling-circle replication: recent developments. Mol Microbiol 37(3):477–484

    Article  CAS  PubMed  Google Scholar 

  12. Khan SA (2005) Plasmid rolling-circle replication: highlights of two decades of research. Plasmid 53(2):126–136

    Article  CAS  PubMed  Google Scholar 

  13. Kim SW, Jeong EJ, Kang HS, Tak JI, Bang WY, Heo JB, Bahk JD (2006) Role of RepB in the replication of plasmid pJB01 isolated from Enterococcus faecium JC1. Plasmid 55(2):99–113

    Article  CAS  PubMed  Google Scholar 

  14. Kleerebezem M, Boekhorst J, van Kranenburg R, Molenaar D, Kuipers OP, Leer R, Stiekema W (2003) Complete genome sequence of Lactobacillus plantarum WCFS1. Proc Natl Acad Sci 100(4):1990–1995

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Klein JR, Ulrich C, Plapp R (1993) Characterization and sequence analysis of a small cryptic plasmid from Lactobacillus curvatus LTH683 and its use for construction of new Lactobacillus cloning vectors. Plasmid 30(1):14–29

    Article  CAS  PubMed  Google Scholar 

  16. Lee CL, Ow DSW, Oh SKW (2006) Quantitative real-time polymerase chain reaction for determination of plasmid copy number in bacteria. J Microbiol Methods 65(2):258–267

    Article  CAS  PubMed  Google Scholar 

  17. Leenhouts KJ, Tolner B, Bron S, Kok J, Venema G, Seegers JF (1991) Nucleotide sequence and characterization of the broad-host-range lactococcal plasmid pWVO1. Plasmid 26(1):55–66

    Article  CAS  PubMed  Google Scholar 

  18. Li R, Zhai Z, Yin S, Huang Y, Wang Q, Luo Y, Hao Y (2009) Characterization of a rolling-circle replication plasmid pLR1 from Lactobacillus plantarum LR1. Curr Microbiol 58(2):106–110

    Article  CAS  PubMed  Google Scholar 

  19. Moscoso M, Eritja R, Espinosa M (1997) Initiation of replication of plasmid pMV158: mechanisms of DNA strand-transfer reactions mediated by the initiator RepB protein. J Mol Biol 268(5):840–856

    Article  CAS  PubMed  Google Scholar 

  20. Pan Q, Zhang L, Li J, Chen T, Chen W, Wang G, Yin J (2011) Characterization of pLP18, a novel cryptic plasmid of Lactobacillus plantarum PC518 isolated from Chinese pickle. Plasmid 65(3):204–209

    Article  CAS  PubMed  Google Scholar 

  21. Rogosa M, Mitchell JA, Wiseman RF (1951) A selective medium for the isolation and enumeration of oral and fecal lactobacilli. J Bacteriol 62(1):132

    CAS  PubMed  PubMed Central  Google Scholar 

  22. Ruiz-Barba JL, Piard JC, Jiménez-Díaz R (1991) Plasmid profiles and curing of plasmids in Lactobacillus plantarum strains isolated from green olive fermentations. J Appl Bacteriol 71(5):417–421

    Article  CAS  PubMed  Google Scholar 

  23. Sánchez C, Mayo B (2003) Sequence and analysis of pBM02, a novel RCR cryptic plasmid from Lactococcus lactis subsp. cremoris P8-2-47. Plasmid 49(2):118–129

    Article  PubMed  Google Scholar 

  24. Seegers JF, Meijer WJJ, Venema G, Bron S, Zhao AC, Khan SA (1995) Structural and functional analysis of the single-strand origin of replication from the lactococcal plasmid pWV01. Mol Gen Genet MGG 249(1):43–50

    Article  CAS  PubMed  Google Scholar 

  25. Shareck J, Choi Y, Lee B, Miguez CB (2004) Cloning vectors based on cryptic plasmids isolated from lactic acid bacteria: their characteristics and potential applications in biotechnology. Crit Rev Biotechnol 24(4):155–208

    Article  CAS  PubMed  Google Scholar 

  26. Solar G, Moscoso M, Espinosa M (1993) Rolling circle-replicating plasmids from gram-positive and gram-negative bacteria: a wall falls. Mol Microbiol 8(5):789–796

    Article  PubMed  Google Scholar 

  27. Te Riele H, Michel B, Ehrlich SD (1986) Single-stranded plasmid DNA in Bacillus subtilis and Staphylococcus aureus. Proc Natl Acad Sci 83(8):2541–2545

    Article  Google Scholar 

  28. Van Kranenburg R, Golic N, Bongers R, Leer RJ, De Vos WM, Siezen RJ, Kleerebezem M (2005) Functional analysis of three plasmids from Lactobacillus plantarum. Appl Environ Microbiol 71(3):1223–1230

    Article  PubMed  PubMed Central  Google Scholar 

  29. Yang EJ, Chang HC (2009) Analysis of pYC2, a cryptic plasmid in Lactobacillus sakei BM5 isolated from kimchi. Biotechnol Lett 31(1):123–130

    Article  CAS  PubMed  Google Scholar 

  30. Yin S, Hao Y, Zhai Z, Li R, Huang Y, Tian H, Luo Y (2008) Characterization of a cryptic plasmid pM4 from Lactobacillus plantarum M4. FEMS Microbiol Lett 285(2):183–187

    Article  CAS  PubMed  Google Scholar 

  31. Zhou H, Hao Y, Xie Y, Yin S, Zhai Z, Han B (2010) Characterization of a rolling-circle replication plasmid pXY3 from Lactobacillus plantarum XY3. Plasmid 64(1):36–40

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The work was supported by Chinese Universities Scientific Fund (2015SP005).

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Authors and Affiliations

  1. The Innovation Centre of Food Nutrition and Human Health (Beijing), College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People’s Republic of China

    Fazheng Ren & Yanling Hao

  2. Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality, Beijing, 100083, People’s Republic of China

    Xvchuan Ma, Jinhua Li, Yao Xiong, Zhengyuan Zhai & Yanling Hao

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  1. Xvchuan Ma
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  2. Jinhua Li
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Correspondence to Yanling Hao.

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Ma, X., Li, J., Xiong, Y. et al. Characterization of a Rolling-Circle Replication Plasmid pM411 from Lactobacillus plantarum 1–3. Curr Microbiol 73, 820–826 (2016). https://doi.org/10.1007/s00284-016-1124-7

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  • DOI: https://doi.org/10.1007/s00284-016-1124-7

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