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Comparative Analysis of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) of Streptococcus thermophilus St-I and its Bacteriophage-Insensitive Mutants (BIM) Derivatives

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Abstract

The CRISPR-Cas (CRISPR together with CRISPR-associated proteins) modules are the adaptive immune system, acting as an adaptive and heritable immune system in bacteria and archaea. CRISPR-based immunity acts by integrating short virus sequences in the cell’s CRISPR locus, allowing the cell to remember, recognize, and clear infections. In this study, the homology of CRISPRs sequence in BIMs (bacteriophage-insensitive mutants) of Streptococcus thermophilus St-I were analyzed. Secondary structures of the repeats and the PAMs (protospacer-associated motif) of each CRISPR locus were also predicted. Results showed that CRISPR1 has 27 repeat-spacer units, 5 of them had duplicates; CRISPR2 has one repeat-spacer unit; CRISPR3 has 28 repeat-spacer units. Only BIM1 had a new spacer acquisition in CRISPR3, while BIM2 and BIM3 had no new spacers’ insertion, thus indicating that while most CRISPR1 were more active than CRISPR3, new spacer acquisition occurred just in CRSPR3 in some situations. These findings will help establish the foundation for the study of CRSPR-Cas systems in lactic acid bacteria.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Grant Number: 31171717) and the Special Program for Public Welfare Industry of the Ministry of Agriculture, China (Grant Number: 201203009).

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Authors and Affiliations

  1. Key Laboratory of Dairy Science-Ministry of Education (KLDS-MOE), College of Food Sciences, Northeast Agricultural University (NEAU), Harbin, 150030, Heilongjiang Province, China

    Wan Li, Xin Bian, Smith Etareri Evivie & Gui-cheng Huo

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  1. Wan Li
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  2. Xin Bian
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  3. Smith Etareri Evivie
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  4. Gui-cheng Huo
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Correspondence to Gui-cheng Huo.

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Li, W., Bian, X., Evivie, S.E. et al. Comparative Analysis of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) of Streptococcus thermophilus St-I and its Bacteriophage-Insensitive Mutants (BIM) Derivatives. Curr Microbiol 73, 393–400 (2016). https://doi.org/10.1007/s00284-016-1076-y

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  • DOI: https://doi.org/10.1007/s00284-016-1076-y

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