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Differential Response of Extracellular Proteases of Trichoderma Harzianum Against Fungal Phytopathogens

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Abstract

In the present study, production of extracellular proteases by Trichoderma harzianum was evaluated based on the relative gene expression and spectrophotometric assay. The fungal isolates were grown in Czapek Dox Broth medium supplemented with deactivated mycelium of plant fungal pathogens such as Fusarium oxysporum, Colletotrichum capsici, Gloeocercospora sorghi, and Colletotrichum truncatum. The maximum protease activity was detected after 48 h of incubation against Colletotrichum spp. Similarly in qRT-PCR, the relative gene expression of four proteases varied from 48 to 96 h against host pathogens in a time-independent manner. Among proteases, statistically significant upregulation of asp, asp, and srp was observed against Colletotrichum spp., followed by F. oxysporum. But in the case of pepM22, maximum upregulation was observed against F. oxysporum. The variation in enzyme assay and qRT-PCR of proteases at different time intervals against various fungal phytopathogens could be due to the limitation of using casein as a substrate for all types of proteases or protease-encoding transcripts selected for qRT-PCR, which may not be true representative of total protease activity.

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Acknowledgments

The author is thankful to the Department of Science and Technology, New Delhi for providing funds under DST-FAST Track young scientist scheme (Award Letter No. SB/FT/LS-365/2012). The corresponding author also acknowledges the Department of Plant Pathology, CSK-HPKV, for providing four phytopathogenic fungi used in the present study.

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Authors and Affiliations

  1. Department of Plant Pathology, CSK-Himachal Pradesh Agricultural University, CSK-HPKV, Palampur, 176062, India

    Vivek Sharma & Prem N. Sharma

  2. Department of Veterinary Microbiology, CSK-Himachal Pradesh Agricultural University, Palampur, 176062, India

    Richa Salwan

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  1. Vivek Sharma
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  2. Richa Salwan
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Correspondence to Vivek Sharma.

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Sharma, V., Salwan, R. & Sharma, P.N. Differential Response of Extracellular Proteases of Trichoderma Harzianum Against Fungal Phytopathogens. Curr Microbiol 73, 419–425 (2016). https://doi.org/10.1007/s00284-016-1072-2

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  • DOI: https://doi.org/10.1007/s00284-016-1072-2

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