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Spongiiferula fulva gen. nov., sp. nov., a Bacterium of the Family Flavobacteriaceae Isolated from a Marine Sponge

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Abstract

A Gram stain-negative, strictly aerobic, brown-pigmented, non-motile, rod-shaped, chemoheterotrophic bacterial strain-designated A6F-119T was isolated from a marine sponge (Rhabdastrella sp.). Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the new strain represented a member of the family Flavobacteriaceae of the phylum Bacteroidetes and that it showed highest sequence similarity (93 %) to Tenacibaculum maritimum NBRC 15946T. The strain could be differentiated phenotypically from the recognized members of the family Flavobacteriaceae. The DNA G + C content of strain A6F-119T was determined to be 30.8 mol%; MK-6 was identified as the major menaquinone; and the presence of iso-C15:0, iso-C17:0 3-OH, and C16:1 ω7c and/or C16:1 ω6c as the major (>10 %) cellular fatty acids. A polar lipid profile was present consisting of phosphatidylethanolamine, an unidentified aminolipid, and three unidentified lipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel genus for which the name Spongiiferula fulva gen. nov., sp. nov. is proposed. The type strain of S. fulva is A6F-119T (= KCTC 42752T = NBRC 111402T)

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Acknowledgments

We thank Professor Patricia Bergquist (University of Auckland, New Zealand) for taxonomic analysis of the sponge species. This work was supported by the New Energy and Industrial Technology Development Organization (NEDO), and in part by a research grant (2009–2011) of the Institute for Fermentation, Osaka, Japan.

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Author notes

  1. Kyoko Adachi

    Present address: Basic Research Laboratories, Nippon Steel & Sumikin Chemical Co., Ltd., 1-Tsukiji, Kisarazu, Chiba, 292-0835, Japan

Authors and Affiliations

  1. College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-gu, Daegu, 42601, Republic of Korea

    Jaewoo Yoon

  2. Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate, 026-0001, Japan

    Kyoko Adachi

  3. Marine Biosciences Kamaishi Research Laboratory, Kitasato University, 160-4, Utou, Okirai, Sanriku-cho, Ofunato, Iwate, 022-0101, Japan

    Hiroaki Kasai

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Correspondence to Jaewoo Yoon.

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Electronic Supplementary Material

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Supplementary Fig. 1

A specimen of marine sponge Rhabdastrella sp. Supplementary material 1 (PPTX 83 kb)

Supplementary Fig. 2

Thin-layer chromatograms showing the total polar lipid compositions of A6F-119T. Total polar lipids were detected by spraying the plate with molybdophosphoric acid, molybdenun blue, α-naphthol, and ninhydrin. PE: phosphatidylenthanolamine, UAL: unidentified aminolipid, UL: unidentified lipid. Supplementary material 2 (PPTX 742 kb)

Supplementary Fig. 3

Negative results from the API 20E, API 50CH, and API ZYM strips. Supplementary material 3 (PPTX 44 kb)

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Yoon, J., Adachi, K. & Kasai, H. Spongiiferula fulva gen. nov., sp. nov., a Bacterium of the Family Flavobacteriaceae Isolated from a Marine Sponge. Curr Microbiol 73, 15–21 (2016). https://doi.org/10.1007/s00284-016-1022-z

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  • DOI: https://doi.org/10.1007/s00284-016-1022-z

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