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Pantoea pleuroti sp. nov., Isolated from the Fruiting Bodies of Pleurotus eryngii

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Abstract

Four Gram-negative-staining, facultatively anaerobic bacterial isolates were obtained from the fruiting bodies of the edible mushroom Pleurotus eryngii showing symptoms of bacterial blight disease in Beijing, China. Nearly complete 16S rRNA gene sequencing placed these isolates in the genus Pantoea. Multilocus sequence analysis based on the partial sequences of atpD, gyrB, infB and rpoB revealed Pantoea agglomerans as their closest phylogenetic relatives. DNA–DNA hybridization and phenotypic tests confirmed the classification of the new isolates as a novel species. The name Pantoea pleuroti sp. nov. [type strain KCTC 42084T = CGMCC 1.12894T = JZB 2120015T] is proposed.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (NSFC 31401929), Beijing Academy of Agriculture and Forestry Sciences (KJCX20150401) and Beijing Innovative Team of Edible Mushrooms (Grant No. PXM2013-036204-00069).

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Authors and Affiliations

  1. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing, 100097, China

    Yuanwei Ma, Chengbo Rong, Yu Liu, Shouxian Wang & Feng Xu

  2. College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China

    Yuanwei Ma

  3. Beijing Zhengxinglong Biotechnology Co., Ltd, Beijing, 102211, China

    Yonggang Yin

  4. State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China

    Sanfeng Chen

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  1. Yuanwei Ma
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  2. Yonggang Yin
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  5. Yu Liu
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  7. Feng Xu
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Correspondence to Feng Xu.

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Supplementary Fig. S1

Neighbour-joining tree based on nearly-complete 16S rRNA gene sequences of Pantoea pleuroti and its phylogenetically related species. Species belonging to the genus Tatumella were included as outgroups. Bootstrap percentage values as obtained from 1000 resamplings of the date set are given at the nodes of the tree. The scale bar indicates the numbers of substitutions per site. (PDF 238 kb)

Supplementary Fig. S2

Maximum parsimony consensus tree based on concatenated housekeeping gene sequences of Pantoea strains shows the phylogenetic placement of Pantoea pleuroti. Species belonging to the genus Tatumella were included as outgroups. Bootstrap percentage values of NJ, ML and MP respectively, obtained from 1000 resamplings of the date set, and are given at the nodes of the tree. Only values above 70% are showed here. (PDF 254 kb)

Supplementary material 3 (DOCX 16 kb)

Supplementary material 4 (DOCX 17 kb)

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Ma, Y., Yin, Y., Rong, C. et al. Pantoea pleuroti sp. nov., Isolated from the Fruiting Bodies of Pleurotus eryngii . Curr Microbiol 72, 207–212 (2016). https://doi.org/10.1007/s00284-015-0940-5

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  • DOI: https://doi.org/10.1007/s00284-015-0940-5

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