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A Comparison of Anammox Bacterial Abundance and Community Structures in Three Different Emerged Plants-Related Sediments

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Abstract

Quantitative polymerase chain reaction (qPCR) assays and 16S rRNA gene clone libraries were used to document the abundance, diversity and community structure of anaerobic ammonia-oxidising (anammox) bacteria in the rhizosphere and non-rhizosphere sediments of three emergent macrophyte species (Iris pseudacorus, Thalia dealbata and Typha orientalis). The qPCR results confirmed the existence of anammox bacteria (AMX) with observed log number of gene copies per dry gram sediment ranging from 5.00 to 6.78. AMX was more abundant in T. orientalis-associated sediments than in the other two plant species. The I. pseudacorus- and T. orientalis-associated sediments had higher Shannon diversity values, indicating higher AMX diversity in these sediments. Based on the 16S rRNA gene, Candidatus ‘Brocadia’, Candidatus ‘Kuenenia’, Candidatus ‘Jettenia’ and new clusters were observed with the predominant Candidatus ‘Kuenenia’ cluster. The I. pseudacorus-associated sediments contained all the sequences of the C. ‘Jettenia’ cluster. Sequences obtained from T. orientalis-associated sediments contributed more than 90 % sequences in the new cluster, whereas none was found from I. pseudacorus. The new cluster was distantly related to known sequences; thus, this cluster was grouped outside the known clusters, indicating that the new cluster may be a new Planctomycetales genus. Further studies should be undertaken to confirm this finding.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (51109097), Jiangsu Province Basic Research Program (Natural Science Foundation Funded Project) (BK2011520), China Postdoctoral Science Foundation Funded Project (2012T50464) and Students Scientific Research Funded Project of Jiangsu University (12A258). The authors would like to thank the financial support.

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Authors and Affiliations

  1. Department of Environment Engineering, School of the Environment and Safety Engineering, Jiangsu University, 301, Xuefu Road, Zhenjiang, 212013, Jiangsu, People’s Republic of China

    Jinyu Chu, Jinping Zhang, Xiaohong Zhou & Yimin Li

  2. Faculty of Environmental and Municipal Engineering, Henan University of Urban Construction, Longxiang Road, Pingdingshan, 467036, Henan, China

    Biao Liu

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  1. Jinyu Chu
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  2. Jinping Zhang
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  3. Xiaohong Zhou
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  4. Biao Liu
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  5. Yimin Li
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Correspondence to Xiaohong Zhou.

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Chu, J., Zhang, J., Zhou, X. et al. A Comparison of Anammox Bacterial Abundance and Community Structures in Three Different Emerged Plants-Related Sediments. Curr Microbiol 71, 421–427 (2015). https://doi.org/10.1007/s00284-015-0851-5

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