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Sialidase Production and Genetic Diversity in Clostridium perfringens Type A Isolated from Chicken with Necrotic Enteritis in Brazil

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Abstract

The sialidase activity and genetic diversity of 22 Clostridium perfringens strains isolated from chickens with necrotic enteritis were determined. Sialidase activity was detected in 86.4 % of the strains. All C. perfringens showed a high value of similarity (>96 %), and they were grouped into seven clusters clearly separated from the other reference bacterial strains. From these clusters four patterns were defined in accordance with their phenotypic (sialidase production and antibiotic resistance profile) and genotypic (presence of nanI and nanJ genes) characteristics. Our results showed heterogeneity among strains, but they were genotypically similar, and it is suggested further studies are needed to better understand the pathogenesis of necrotic enteritis.

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Acknowledgments

The authors thank Marcia Harumi Fukugaiti for her technical support. During the course of this work VN was supported by FAPESP fellowship (2009/03792-0). This study was supported by Grant from Conselho Nacional Desenvolvimento Científico e Tecnológico (CNPq Proc. No. 143387/2008-1) and FAPESP (10/52417-4 and 13/17739-9).

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The authors declare no conflict of interest.

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Correspondence to Mario J. Avila-Campos.

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Luis A. Llanco and Viviane Nakano have contributed equally to the work.

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Llanco, L.A., Nakano, V. & Avila-Campos, M.J. Sialidase Production and Genetic Diversity in Clostridium perfringens Type A Isolated from Chicken with Necrotic Enteritis in Brazil. Curr Microbiol 70, 330–337 (2015). https://doi.org/10.1007/s00284-014-0722-5

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  • DOI: https://doi.org/10.1007/s00284-014-0722-5

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