Abstract
In this study, we classified the five strains (ChDC F128T, ChDC F145, ChDC F174, ChDC F206, and ChDC F300) as a novel species of genus Fusobacterium by DNA–DNA hybridization and multi-locus phylogenetic analysis (MLPA), based on a single sequence (24,715 bp) of 22 concatenated housekeeping genes, with morphological and chemotaxonomic characteristics. DNA–DNA hybridization data showed that the values of genomic relatedness between ChDC F128T and each of the other novel strains were ranged from 79.0 to 82.6 %, while those of genomic relatedness between ChDC F128T and type strain of each of subspecies of F. nucleatum or Fusobacterium periodonticum were ranged from 40.9 to 54.4 %. MLPA revealed that the 5 strains were clustered as one group and clearly discriminated with F. nucleatum and F. periodonticum with 100 % bootstrap value. The DNA G+C content of the five novel strains were ranged from 26.9 to 27.0 mol%. The cellular fatty acid analysis of clinical isolates and type strains revealed C14:0, C16:0, and cis-9 C16:1 as the major fatty acids. The cell wall peptidoglycan of the 5 strains was comprised of meso-lanthionine. These results show that the 5 strains are novel species and belong to the genus Fusobacterium. Strain ChDC F128T (=KCOM 1249T = KCTC 5108T = JCM 30218T) is suggested to be the type strain of a novel species of genus Fusobacterium, for which the name Fusobacterium hwasookii sp. nov. is proposed.
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Acknowledgments
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and future Planning (NRF-2012R1A1A2003324) and in part by a Grant TGM0721311 funded by the Ministry of Trade, Industry and Energy.
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Eugene Cho and Soon-Nang Park contributed equally to this study.
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Supplementary Fig. S1. Neighbor-joining phylogenetic trees based on a single sequence (24,715 bp) by concatenating 22 housekeeping genes. The stability of phylogenetic trees was assessed by a bootstrap analysis of 1,000 replicates by using MEGA version 6.06 [17]. Bar indicates 0.01 changes per nucleotide position
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Supplementary Fig. S2. Scanning electron micrograph of strains (a) ChDC F128T, (b) ChDC F145, (c) ChDC F174, (d) ChDC F206, and (e) ChDC F300. The scale bars represent 30 μm (a), 10 μm (b), 100 μm (c), 20 μm (d), and 20 μm (e)
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Supplementary Fig. S3. Thin-layer chromatography of acid hydrolysates of peptidoglycan. Strains: 1, strain ChDC F128T; 2, F. nucleatum subsp. nucleatum ATCC25586T; 3, F. nucleatum subsp. polymorphum ATCC10953T; 4, F. nucleatum subsp. vincentii ATCC49256T; 5, F. nucleatum subsp. animalis ATCC51191T; 6, strain ChDC F128T; 7, F. periodonticum ATCC33693T. Arrow indicates meso-lanthionine (Lan)
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Supplementary Fig. S4. Polar lipid profile of ChDC F128T by two-dimensional thin-layer chromatography. Abbreviations: PE, Phosphatidylethanolamine; DPG, Diphosphatidylglycerol; PG, Phosphatidylglycerol; PL, unknown phospholipid; AL, unknown aminolipid; PN, unknown aminophospholipid; L, unknown polar lipid
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Cho, E., Park, SN., Lim, Y.K. et al. Fusobacterium hwasookii sp. nov., Isolated from a Human Periodontitis Lesion. Curr Microbiol 70, 169–175 (2015). https://doi.org/10.1007/s00284-014-0692-7
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DOI: https://doi.org/10.1007/s00284-014-0692-7