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Acid Resistance Contributes to the High-Pressure Carbon Dioxide Resistance of Escherichia coli K-12

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Abstract

Effect of deletion of acid resistant genes of E. coli on the high-pressure carbon dioxide (HPC) resistance was investigated. Genes coding amino acid decarboxylases, such as lysine, arginine, and glutamate decarboxylase, were found to contribute to HPC resistance. Protonophore-treated cells showed hypersensitivity to HPC, confirming that HPC induced cytoplasm acidification and exerted severe damage on cells by intrusion of gaseous carbon dioxide into cytoplasm.

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Acknowledgments

We would like to thank the National Institute of Genetics (Shizuoka) and National BioResource Project (NIG, Japan): E. coli for giving us the E. coli strains and plasmids used in this study.

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Authors and Affiliations

  1. Department of Food Bioscience and Biotechnology College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-8510, Japan

    Soichi Furukawa, Junji Shimazaki, Kazumichi Kawaharada, Tsukasa Matsuda, Hiroki Aoyagi, Hidekazu Wakabayashi, Hirokazu Ogihara, Makari Yamasaki & Yasushi Morinaga

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  1. Soichi Furukawa
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  2. Junji Shimazaki
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  3. Kazumichi Kawaharada
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  4. Tsukasa Matsuda
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  5. Hiroki Aoyagi
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  6. Hidekazu Wakabayashi
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  7. Hirokazu Ogihara
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  8. Makari Yamasaki
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  9. Yasushi Morinaga
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Corresponding authors

Correspondence to Soichi Furukawa, Makari Yamasaki or Yasushi Morinaga.

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Furukawa, S., Shimazaki, J., Kawaharada, K. et al. Acid Resistance Contributes to the High-Pressure Carbon Dioxide Resistance of Escherichia coli K-12. Curr Microbiol 70, 1–5 (2015). https://doi.org/10.1007/s00284-014-0674-9

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  • DOI: https://doi.org/10.1007/s00284-014-0674-9

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