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Spirosoma radiotolerans sp. nov., a Gamma-Radiation-Resistant Bacterium Isolated from Gamma Ray-Irradiated Soil

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Abstract

A Gram-negative, short-rod-shaped bacterial strain with gliding motility, designated as DG5AT, was isolated from a rice field soil in South Korea. Phylogenic analysis using 16S rRNA gene sequence of the new isolate showed that strain DG5AT belong to the genus Spirosoma in the family Spirosomaceae, and the highest sequence similarities were 95.5 % with Spirosoma linguale DSM 74T, 93.4 % with Spirosoma rigui WPCB118T, 92.8 % with Spirosoma luteum SPM-10T, 92.7 % with Spirosoma spitsbergense SPM-9T, and 91.9 % with Spirosoma panaciterrae Gsoil 1519T. Strain DG5AT revealed resistance to gamma and UV radiation. Chemotaxonomic data showed that the most abundant fatty acids were summed feature C16:1 ω7c/C16:1 ω6c (36.90 %), C16:1 ω5c (29.55 %), and iso-C15:0 (14.78 %), and the major polar lipid was phosphatidylethanolamine (PE). The DNA G+C content of strain DG5AT was 49.1 mol%. Together, the phenotypic, phylogenetic, and chemotaxonomic data supported that strain DG5AT presents a novel species of the genus Spirosoma, for which the name Spirosoma radiotolerans sp. nov., is proposed. The type strain is DG5AT (=KCTC 32455T = JCM19447T).

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Acknowledgments

This work was supported by a special research grant from Seoul Women’s University (2014). The authors are grateful to Dr. Jean Euzéby for help with etymology.

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Authors and Affiliations

  1. Department of Bio & Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul, 139-774, Republic of Korea

    Jae-Jin Lee, Sathiyaraj Srinivasan, So Il Bae, Kyoung Ryun Park, Ji-Hee Han, Se-Hee Park, Bo-min Joo, Sol-Ji Park & Myung Kyum Kim

  2. Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, 580-185, Republic of Korea

    Sangyong Lim, Minho Joe & Seonghun Im

  3. Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University, Gwangju, 500-757, Republic of Korea

    Seonghun Im

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  1. Jae-Jin Lee
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Correspondence to Myung Kyum Kim.

Additional information

The NCBI GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DG5A (=KCTC 32455 = JCM19447 ) is KF303585.

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Lee, JJ., Srinivasan, S., Lim, S. et al. Spirosoma radiotolerans sp. nov., a Gamma-Radiation-Resistant Bacterium Isolated from Gamma Ray-Irradiated Soil. Curr Microbiol 69, 286–291 (2014). https://doi.org/10.1007/s00284-014-0584-x

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