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Bombyx mori Nucleopolyhedrovirus ORF101 Encodes a Budded Virus Envelope Associated Protein

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Abstract

Orf101 (Bm101) of Bombyx mori nucleopolyhedrovirus (BmNPV) is a highly conserved gene in lepidopteran nucleopolyhedroviruses, but its function remains unknown. In this study, Bm101 was characterized. Transcripts of Bm101 were detected from 24 through 96 h post infection (h p.i.) by RT-PCR. The corresponding protein was also detected from 24 to 96 h p.i. in BmNPV-infected BmN cells by Western blot analysis using a polyclonal antibody against Bm101. Western blot assay of occlusion-derived virus and budded virus (BV) preparations revealed that Bm101 encodes a 28-kDa structural protein that is associated with BV and is located in the envelope fraction of budded virions. In addition, confocal analysis showed that the protein was localized in the cytosol and cytoplasmic membrane in virus-infected cells. In conclusion, the available data suggest that Bm101 is a functional ORF of BmNPV and encodes a protein expressed in the late stage of the infection cycle that is associated with the BV envelope.

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References

  1. Appel RD, Bairoch A, Hochstrasser DF (1994) A new generation of information retrieval tools for biologists: the example of the ExPASy WWW server. Trends Biochem Sci 19:258–260

    Article  CAS  PubMed  Google Scholar 

  2. Blissard GW, Rohrmann GF (1989) Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein gene of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 170:537–555

    Article  CAS  PubMed  Google Scholar 

  3. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  4. Braunagel SC, Summers MD (1994) Autographa californica nuclear polyhedrosis virus, PDV, and ECV viral envelopes and nucleocapsids: structural proteins, antigens, lipid and fatty acid profiles. Virology 202:315–328

    Article  CAS  PubMed  Google Scholar 

  5. Gomi S, Majima K, Maeda S (1999) Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus. J Gen Virol 80(Pt 5):1323–1337

    CAS  PubMed  Google Scholar 

  6. Granados RR, Lawler KA (1981) In vivo pathway of Autographa californica baculovirus invasion and infection. Virology 108:297–308

    Article  CAS  PubMed  Google Scholar 

  7. Harlow E, Lane D (1988) Antibodies: a laboratory manual, 1st edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  8. Hasnain SE, Jain A, Habib S et al (1997) Involvement of host factors in transcription from baculovirus very late promoters—a review. Gene 190:113–118

    Article  CAS  PubMed  Google Scholar 

  9. Hefferon KL (2004) Baculovirus late expression factors. J Mol Microbiol Biotechnol 7:89–101

    Article  CAS  PubMed  Google Scholar 

  10. Herniou EA, Olszewski JA, Cory JS et al (2003) The genome sequence and evolution of baculoviruses. Annu Rev Entomol 48:211–234

    Article  CAS  PubMed  Google Scholar 

  11. Keddie BA, Aponte GW, Volkman LE (1989) The pathway of infection of Autographa californica nuclear polyhedrosis virus in an insect host. Science 243:1728–1730

    Article  CAS  PubMed  Google Scholar 

  12. Lin F, Chen K, Xia H et al (2011) Characterization of a late gene, ORF134, from Bombyx mori nucleopolyhedrovirus. Intervirology 54:113–121

    Article  CAS  PubMed  Google Scholar 

  13. Lu A, Miller LK (1995) The roles of eighteen baculovirus late expression factor genes in transcription and DNA replication. J Virol 69:975–982

    CAS  PubMed Central  PubMed  Google Scholar 

  14. O’Reilly DR, Lk Miller, Luckow VE (1992) Baculovirus expression vectors: a laboratory manual. Freeman, NewYork

    Google Scholar 

  15. Pearson MN, Groten C, Rohrmann GF (2000) Identification of the lymantria dispar nucleopolyhedrovirus envelope fusion protein provides evidence for a phylogenetic division of the Baculoviridae. J Virol 74:6126–6131

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  16. Rahman MM, Gopinathan KP (2003) Characterization of the gene encoding the envelope fusion glycoprotein GP64 from Bombyx mori nucleopolyhedrovirus. Virus Res 94:45–57

    Article  CAS  PubMed  Google Scholar 

  17. Slack J, Arif BM (2007) The baculoviruses occlusion-derived virus: virion structure and function. Adv Virus Res 69:99–165

    Article  CAS  PubMed  Google Scholar 

  18. Summers MD, Smith GE (1975) Trichoplusia ni granulosis virus granulin: a phenol-soluble, phosphorylated protein. J Virol 16:1108–1116

    CAS  PubMed Central  PubMed  Google Scholar 

  19. Volkman LE, Goldsmith PA (1985) Mechanism of neutralization of budded Autographa californica nuclear polyhedrosis virus by a monoclonal antibody: inhibition of entry by adsorptive endocytosis. Virology 143:185–195

    Article  CAS  PubMed  Google Scholar 

  20. Wang D, An SH, Guo ZJ et al (2005) Characterization of Helicoverpa armigera nucleopolyhedrovirus orf33 that encodes a novel budded virion derived protein, BV-e31. Arch Virol 150:1505–1515

    Article  CAS  PubMed  Google Scholar 

  21. Wf IJ, Westenberg M, Goldbach RW et al (2000) A novel baculovirus envelope fusion protein with a proprotein convertase cleavage site. Virology 275:30–41

    Article  Google Scholar 

  22. Xu HJ, Liu YH, Yang ZN et al (2006) Characterization of ORF39 from Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus, the gene containing RNA recognition motif. J Biochem Mol Biol 39:263–269

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (31100118, 31370790), Natural Science Foundation of Jiangsu Province (BK2011495), Natural Science Foundation of the Higher Education Institutions of Jiangsu Province (09KJB180001), and Startup Scientific Research Fund from Jiangsu University for Advanced Professionals (09JDG006).

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Correspondence to Huiqing Chen.

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Chen, H., Li, M., Huang, G. et al. Bombyx mori Nucleopolyhedrovirus ORF101 Encodes a Budded Virus Envelope Associated Protein. Curr Microbiol 69, 158–163 (2014). https://doi.org/10.1007/s00284-014-0570-3

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  • DOI: https://doi.org/10.1007/s00284-014-0570-3

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