Abstract
A novel Gram-negative, orange-pigmented bacterial strain JLT2008T was isolated from the surface seawater of the Western Pacific and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JLT2008T belonged to the genus Erythrobacter, sharing the highest similarity (96.6 %) with Erythrobacter gangjinensis K7-2T and the lowest similarity (94.9 %) with Erythrobacter litoralis DSM 8509T. Strain JLT2008T did not contain bacteriochlorophyll a, and the predominant respiratory lipoquinone was ubiquinone-10. The major fatty acids were C18:1 ω7c, C16:0, C16:1 ω7c/C16:1 ω6c. The prominent polar lipids were sphingoglycolipid, phosphatidylethanolamine, and phosphatidylglycerol. The genomic G + C content was 60.1 mol %. Based on the polyphasic taxonomic data, a novel species within the genus Erythrobacter, and with the name Erythrobacter westpacificensis sp. nov., is proposed. The type strain is JLT2008T (=CGMCC 1.10993T = JCM 18014T).
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References
Anzai Y, Kim H, Park JY, Wakabayashi H, Oyaizu H (2000) Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50:1563–1589
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK, Lim YW (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261
Denner EBM, Vybiral D, Kobl M, Peter K, Velimirov B (2002) Erythrobacter citreus sp. nov., a yellow-pigmented bacterium that lacks bacteriochlorophyll a, isolated from the western Mediterranean Sea. Int J Syst Evol Microbiol 52:1655–1661
Dong XZ, Cai MY (2001) Determinative manual for routine bacteriology. Scientific Press, Peking, pp 353–412
Embley T (1991) The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Lett Appl Microbiol 13:171–174
Gerhardt P, Murray R, Wood WA, Krieg NR (1994) Methods for general and molecular bacteriology. American society for Microbiology, Washington, DC
Hansen GH, Sørheim R (1991) Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 13:231–241
Ivanova EP, Bowman JP, Lysenko AM, Zhukova NV, Gorshkova NM, Kuznetsova TA, Kalinovskaya NI, Shevchenko LS, Mikhailov VV (2005) Erythrobacter vulgaris sp. nov., a novel organism isolated from the marine invertebrates. Syst Appl Microbiol 28:123–130
Johnson J (1994) Similarity analysis of DNAs. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Method for general and molecular bacteriology. American Society for Microbiology Press, Washington, DC, pp 655–681
Jung YT, Park S, Oh TK, Yoon JH (2011) Erythrobacter marinus sp. nov., isolated from seawater of the Yellow Sea in Korea. Int J Syst Evol Microbiol. doi:10.1099/ijs.0.034702-0
Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment briefings. Bioinformatics 5:150–163
Lee YS, Lee DH, Kahng HY, Kim EM, Jung JS (2010) Erythrobacter gangjinensis sp. nov., a marine bacterium isolated from seawater. Int J Syst Evol Microbiol 60:1413–1417
Lemos ML, Toranzo AE, Barja JL (1985) Modified medium for the oxidation–fermentation test in the identification of marine bacteria. Appl Environ Microbiol 49:1541–1543
Mandel M, Igambi L, Bergendahl J, Dodson ML, Scheltgen E (1970) Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. J Bacteriol 101:333–338
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Inc., Newark
Shiba T, Simidu U (1982) Erythrobacter longus gen. nov., sp. nov., an aerobic bacterium which contains bacteriochlorophyll α. Int J Syst Evol Microbiol 32:211–217
Tindall B (1990) A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130
Tindall B (1990) Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66:199–202
Tindall B, Sikorski J, Smibert R, Krieg N (2007) Phenotypic characterization and principles of comparative systematics. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM, Snyder LR (eds) Methods for general and molecular microbiology, 3rd edn. American Society for Microbiology press, Washington, DC, pp 330–393
Wu H, Lai PY, Lee OO, Zhou X, Miao L, Wang H, Qian PY (2011) Erythrobacter pelagi sp. nov., a novel bacterium in the family Erythrobacteraceae isolated from the Red Sea. Int J Syst Evol Microbiol doi:10.1099/ijs.0.029561-0
Xu M, Xin Y, Yu Y, Zhang J, Zhou Y, Liu H, Tian J, Li Y (2010) Erythrobacter nanhaisediminis sp. nov., isolated from marine sediment of the South China Sea. Int J Syst Evol Microbiol 60:2215–2220
Ying JY, Wang BJ, Dai X, Yang SS, Liu SJ, Liu ZP (2007) Wenxinia marina gen. nov., sp. nov., a novel member of the Roseobacter clade isolated from oilfield sediments of the South China Sea. Int J Syst Evol Microbiol 57:1711–1716
Yoon JH (2005) Erythrobacter seohaensis sp. nov. and Erythrobacter gaetbuli sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 55:71–75
Yoon JH, Kim H, Kim IG, Kang KH, Park YH (2003) Erythrobacter flavus sp. nov., a slight halophile from the East Sea in Korea. Int J Syst Evol Microbiol 53:1169–1174
Yoon JH, Kang KH, Oh TK, Park YH (2004) Erythrobacter aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54:1981–1985
Yoon BJ, Lee DH, Oh DC (2012) Erythrobacter jejuensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol. doi:10.1099/ijs.0.038349-0
Yurkov V, Stackebrandt E, Holmes A, Fuerst JA, Hugenholtz P, Golecki J, Gad’on N, Gorlenko VM, Kompantseva EI, Drews G (1994) Phylogenetic positions of novel aerobic, bacteriochlorophyll a -containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. Int J Syst Evol Microbiol 44:427–434
Yurkov VV, Krieger S, Stackebrandt E, Beatty JT (1999) Citromicrobium bathyomarinum, a novel aerobic bacterium isolated from deep-sea hydrothermal vent plume waters that contains photosynthetic pigment-protein complexes. J Bacteriol 181:4517–4525
Acknowledgments
The authors would like to thank Yingnan Fu for direction of the experiments and Jia Sun for sampling. This work was supported by the NSFC Project (91028001), the SOA (State Oceanic Administration) project (201105021), and the 863 Project (2012AA092003). Rui Zhang was supported by the Fundamental Research Funds for the Central Universities (2011121012). Professor John Hodgkiss of the University of Hong Kong is thanked for his help with English.
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The GenBank accession number for the 16S rRNA gene sequence of strain JLT2008T is JN164701.
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Wei, J., Mao, Y., Zheng, Q. et al. Erythrobacter westpacificensis sp. nov., a Marine Bacterium Isolated From the Western Pacific. Curr Microbiol 66, 385–390 (2013). https://doi.org/10.1007/s00284-012-0287-0
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DOI: https://doi.org/10.1007/s00284-012-0287-0