Current Microbiology

, Volume 62, Issue 1, pp 182–190

The Diversity and Anti-Microbial Activity of Endophytic Actinomycetes Isolated from Medicinal Plants in Panxi Plateau, China

Authors

  • Ke Zhao
    • Department of Microbiology, College of Resource and Environmental SciencesSichuan Agricultural University
  • Petri Penttinen
    • Department of Applied Chemistry and MicrobiologyUniversity of Helsinki
  • Tongwei Guan
    • Department of Microbiology, College of Resource and Environmental SciencesSichuan Agricultural University
    • Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction CorpsTarim University
  • Jing Xiao
    • Key Laboratory of Marine Biogenetic Resources, The Third Institute of OceanographyState Oceanic Administration
  • Qiang Chen
    • Department of Microbiology, College of Resource and Environmental SciencesSichuan Agricultural University
  • Jun Xu
    • Key Laboratory of Marine Biogenetic Resources, The Third Institute of OceanographyState Oceanic Administration
  • Kristina Lindström
    • Department of Applied Chemistry and MicrobiologyUniversity of Helsinki
    • Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction CorpsTarim University
    • Department of Microbiology, College of Resource and Environmental SciencesSichuan Agricultural University
  • Gary A. Strobel
    • Department of Plant SciencesMontana State University
Article

DOI: 10.1007/s00284-010-9685-3

Cite this article as:
Zhao, K., Penttinen, P., Guan, T. et al. Curr Microbiol (2011) 62: 182. doi:10.1007/s00284-010-9685-3

Abstract

Traditional Chinese medicinal plants are sources of biologically active compounds, providing raw material for pharmaceutical, cosmetic and fragrance industries. The endophytes of medicinal plants participate in biochemical pathways and produce analogous or novel bioactive compounds. Panxi plateau in South-west Sichuan in China with its unique geographical and climatological characteristics is a habitat of a great variety of medicinal plants. In this study, 560 endophytic actinomycetes were isolated from 26 medicinal plant species in Panxi plateau. 60 isolates were selected for 16S rDNA-RFLP analysis and 14 representative strains were chosen for 16S rDNA sequencing. According to the phylogenetic analysis, seven isolates were Streptomyces sp., while the remainder belonged to genera Micromonospora, Oerskovia, Nonomuraea, Promicromonospora and Rhodococcus. Antimicrobial activity analysis combined with the results of amplifying genes coding for polyketide synthetase (PKS-I, PKS-II) and nonribosomal peptide synthetase (NRPS) showed that endophytic actinomycetes isolated from medicinal plants in Panxi plateau had broad-spectrum antimicrobial activity and potential natural product diversity, which further proved that endophytic actinomycetes are valuable reservoirs of novel bioactive compounds.

Supplementary material

284_2010_9685_MOESM1_ESM.doc (136 kb)
Supplementary material 1 (DOC 136 kb)

Copyright information

© Springer Science+Business Media, LLC 2010