Article

Current Microbiology

, Volume 62, Issue 1, pp 182-190

The Diversity and Anti-Microbial Activity of Endophytic Actinomycetes Isolated from Medicinal Plants in Panxi Plateau, China

  • Ke ZhaoAffiliated withDepartment of Microbiology, College of Resource and Environmental Sciences, Sichuan Agricultural University
  • , Petri PenttinenAffiliated withDepartment of Applied Chemistry and Microbiology, University of Helsinki
  • , Tongwei GuanAffiliated withDepartment of Microbiology, College of Resource and Environmental Sciences, Sichuan Agricultural UniversityKey Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University
  • , Jing XiaoAffiliated withKey Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography, State Oceanic Administration
  • , Qiang ChenAffiliated withDepartment of Microbiology, College of Resource and Environmental Sciences, Sichuan Agricultural University
  • , Jun XuAffiliated withKey Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography, State Oceanic Administration
  • , Kristina LindströmAffiliated withDepartment of Applied Chemistry and Microbiology, University of Helsinki
  • , Lili ZhangAffiliated withKey Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University Email author 
  • , Xiaoping ZhangAffiliated withDepartment of Microbiology, College of Resource and Environmental Sciences, Sichuan Agricultural University Email author 
    • , Gary A. StrobelAffiliated withDepartment of Plant Sciences, Montana State University

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Abstract

Traditional Chinese medicinal plants are sources of biologically active compounds, providing raw material for pharmaceutical, cosmetic and fragrance industries. The endophytes of medicinal plants participate in biochemical pathways and produce analogous or novel bioactive compounds. Panxi plateau in South-west Sichuan in China with its unique geographical and climatological characteristics is a habitat of a great variety of medicinal plants. In this study, 560 endophytic actinomycetes were isolated from 26 medicinal plant species in Panxi plateau. 60 isolates were selected for 16S rDNA-RFLP analysis and 14 representative strains were chosen for 16S rDNA sequencing. According to the phylogenetic analysis, seven isolates were Streptomyces sp., while the remainder belonged to genera Micromonospora, Oerskovia, Nonomuraea, Promicromonospora and Rhodococcus. Antimicrobial activity analysis combined with the results of amplifying genes coding for polyketide synthetase (PKS-I, PKS-II) and nonribosomal peptide synthetase (NRPS) showed that endophytic actinomycetes isolated from medicinal plants in Panxi plateau had broad-spectrum antimicrobial activity and potential natural product diversity, which further proved that endophytic actinomycetes are valuable reservoirs of novel bioactive compounds.