Current Microbiology

, Volume 61, Issue 5, pp 422-428

First online:

Prevention of Chinese Sacbrood Virus Infection in Apis cerana using RNA Interference

  • Xuejiao LiuAffiliated withSouth China Botanical Garden, Chinese Academy of SciencesGuangdong Entomological Institute
  • , Yi ZhangAffiliated withGuangdong Entomological InstituteSun Yat-sen University
  • , Xun YanAffiliated withGuangdong Entomological Institute
  • , Richou HanAffiliated withGuangdong Entomological Institute Email author 

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Chinese sacbrood virus (CSBV) is the pathogen of Chinese sacbrood disease, which poses a serious threat to honeybee Apis cerana, and tends to cause bee colony and even the whole apiary collapse. Here we report on prevention of CSBV infection by feeding second instar larvae of A. cerana with specific sequences of CSBV double-stranded RNA (dsRNA). Protection of the bee larvae from CSBV by ingestion of CSBV-derived dsRNA was further demonstrated by quantitative real-time PCR (qRT-PCR) and northern blot analysis. The result provides a potential method to protect A. cerana from CSBV infection.