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Prevalence of Community-Occurring Extended Spectrum β-Lactamase-Producing Enterobacteriaceae in Brazil

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Abstract

The occurrence of extended-spectrum-β-lactamase (ESBL)-producing strains in the community was investigated in a private laboratory located in Juiz de Fora, Brazil. All enterobacterial isolates analysed were collected from urine of human patients between the years 2000 and 2002. ESBL production was confirmed by double disk screening, combination disk method, and Etest ESBL strip. The isoelectric point of each β-lactamase was determined in the crude extracts from each isolate. Detection of ESBL genes was performed by polymerase chain reaction and the genetic relatedness of the isolates determined by pulsed-field gel electrophoresis (PFGE). Of the 1,481 isolates, 22 (12 Klebsiella pneumoniae, 7 Escherichia coli, 1 Providencia stuartii, 1 Citrobacter freundii, and 1 Serratia marcescens) were identified as ESBL producers. The frequency of ESBL producers in the community was 1.48%. TEM-type enzymes were identified in 95.4% of the isolates, followed by the SHV type. Seven strains produced CTX-M–type enzymes. This study showed that strains producing multiple β-lactamases are also present in community-acquired bacterial isolates. Multiple strains exhibiting identical PFGE genotypes were found in individual patients indicating a common source of acquisition.

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Acknowledgments

We express our gratitude to the Lemos Laboratórios de Análises Clínicas for supplying the isolates; to Dr. Livermore from the Central Public Health Laboratory, London, UK, for graciously providing controls TEM-1, TEM-3, TEM-6, TEM-9, SHV-1, SHV-2, SHV-4, and SHV-5 ESBL-producing E. coli; to Andrea Penteado for assistance during IEF experiments; and to Joseane C. Ferreira for performing the PFGE assays. This work was financially supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 03/13574-3, São Paulo, Brazil) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior. The project protocol was approved by the Research Ethical Committee of the Faculty of Pharmaceutical Sciences of Ribeirão Preto under the number 26/CEP/FCFRP.

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Authors and Affiliations

  1. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, USP, Ribeirão Preto, São Paulo, 14040-903, Brazil

    Luciene A. R. Minarini, Izabel C. V. Palazzo & Ana Lúcia C. Darini

  2. Laboratório Alerta, Universidade Federal de São Paulo, UNIFESP, São Paulo, 04039-032, Brazil

    Ana C. Gales

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  1. Luciene A. R. Minarini
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  2. Ana C. Gales
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  3. Izabel C. V. Palazzo
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  4. Ana Lúcia C. Darini
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Correspondence to Ana Lúcia C. Darini.

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Minarini, L.A.R., Gales, A.C., Palazzo, I.C.V. et al. Prevalence of Community-Occurring Extended Spectrum β-Lactamase-Producing Enterobacteriaceae in Brazil. Curr Microbiol 54, 335–341 (2007). https://doi.org/10.1007/s00284-006-0307-z

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