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Effect of Low-Level Laser Therapy on Human Adipose-Derived Stem Cells: In Vitro and In Vivo Studies

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Abstract

Background

Low-level laser therapy (LLLT) continues to receive much attention in many clinical fields. Also, LLLT has been used to enhance the proliferation of various cell lines, including stem cells. This study investigated the effect of LLLT on human adipose-derived stem cells (ADSCs) through in vitro and in vivo studies.

Methods

Low-level laser irradiation of cultured ADSCs was performed using a 830 nm Ga–Al–As (gallium–aluminum–arsenide) laser. Then, proliferation of ADSCs was quantified by a cell counting kit-8. In the in vivo study, irradiated ADSCs or non-irradiated ADSCs were transplanted, and then, low-level laser irradiation of each rat was performed as per the protocol. Cell viability was quantified by immunofluorescent staining using the human mitochondria antibody.

Results

In the in vitro study, the laser-irradiated groups showed an increase in absorbance compared to the control group. Also, in the in vivo study, there was a significant increase in the number of human ADSCs in the laser-irradiated groups compared to the control group (p < 0.001).

Conclusions

Our study showed that LLLT could enhance the proliferation and viability of ADSCs. The ADSCs enhanced by LLLT could be applied in various clinical fields. With the use of LLLT, the proliferation and viability of various cells can be enhanced, besides ADSCs.

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Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Eulji University School of Medicine, Eulji General Hospital, Seoul, Republic of Korea

    Kyung Hee Min & Jin Hwan Byun

  2. Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, 82, Gumi-ro 173 Beon-Gil, Bundang-Gu, Seongnam-si, Gyeonggi-do, 463707, Republic of Korea

    Chan Yeong Heo, Hye Yeon Choi & Chang Sik Pak

  3. Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul, Republic of Korea

    Eun Hee Kim

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  1. Kyung Hee Min
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Correspondence to Chan Yeong Heo.

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Min, K.H., Byun, J.H., Heo, C.Y. et al. Effect of Low-Level Laser Therapy on Human Adipose-Derived Stem Cells: In Vitro and In Vivo Studies. Aesth Plast Surg 39, 778–782 (2015). https://doi.org/10.1007/s00266-015-0524-6

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  • DOI: https://doi.org/10.1007/s00266-015-0524-6

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