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Mechano growth factor-E regulates apoptosis and inflammatory responses in fibroblast-like synoviocytes of knee osteoarthritis

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Abstract

Purposes

This study investigated whether mechano growth factor-E (MGF-E) peptide can regulate apoptosis and inflammation responses in fibroblast-like synoviocytes of osteoarthritis (OA).

Methods

A (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) (MTS) assay was performed to evaluate cytotoxic effects of exogenous MGF-E peptide on OA fibroblast-like synoviocytes (OA-FLS). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to check messenger RNA (mRNA) expression levels of lysyl oxidase (LOX) family members (LOX) after OA-FLS treatment using MGF-E peptide. A 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay was performed to identify the influence of MGF-E peptide on proliferation OA-FLS proliferation. Western blot was used to detect biomarkers of endoplasmic reticulum (ER) stress and inflammatory cytokines.

Results

Exogenous MGF-E peptide has no obvious cytotoxic effects on OA-FLS and promotes LOX expression in OA-FLS, induce apoptosis and ER stress and down-regulate protein levels of tumour necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β).

Conclusions

Our results suggest that MGF-E peptide possesses potential anti-inflammatory effects, induces cell apoptosis and facilitates repair of OA-FLS. Therefore, MGF-E peptide may have therapeutic potential in patients with OA.

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Acknowledgments

This work was supported by grants from Innovation and Attracting Talents Program for College and University (“111” Project) (B06023), National Natural Science Foundation of China (11032012, 31270990, and 11172338), Fundamental Research Funds for the Central Universities (106112015CDJRC231206, CDJZR 12238801 and CQDXWL-2014-007). We thank Raina Choi (University of Southern California) for carefully revising the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People’s Republic of China

    Haibin Li, Mingxing Lei, Can Yu, Yonggang Lv, Yang Song & Li Yang

  2. ‘111’ Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing, 400044, China

    Haibin Li, Mingxing Lei, Can Yu, Yonggang Lv, Yang Song & Li Yang

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  1. Haibin Li
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  2. Mingxing Lei
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  3. Can Yu
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  6. Li Yang
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Correspondence to Yang Song or Li Yang.

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Li, H., Lei, M., Yu, C. et al. Mechano growth factor-E regulates apoptosis and inflammatory responses in fibroblast-like synoviocytes of knee osteoarthritis. International Orthopaedics (SICOT) 39, 2503–2509 (2015). https://doi.org/10.1007/s00264-015-2974-5

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  • DOI: https://doi.org/10.1007/s00264-015-2974-5

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