Original Article

European Journal of Nuclear Medicine and Molecular Imaging

, Volume 41, Issue 2, pp 308-321

First online:

Small animal PET imaging of the type 1 cannabinoid receptor in a rodent model for anorexia nervosa

  • Cindy CasteelsAffiliated withDivision of Nuclear Medicine, University Hospital and KU LeuvenMolecular Small Animal Imaging Center (MoSAIC), KU Leuven Email author 
  • , Nathalie GérardAffiliated withDivision of Nuclear Medicine, University Hospital and KU LeuvenMolecular Small Animal Imaging Center (MoSAIC), KU Leuven
  • , Kris van KuyckAffiliated withLaboratory for Experimental Functional Neurosurgery, KU Leuven
  • , Lies PottelAffiliated withDivision of Nuclear Medicine, University Hospital and KU LeuvenMolecular Small Animal Imaging Center (MoSAIC), KU Leuven
  • , Bart NuttinAffiliated withLaboratory for Experimental Functional Neurosurgery, KU Leuven
  • , Guy BormansAffiliated withMolecular Small Animal Imaging Center (MoSAIC), KU LeuvenLaboratory for Radiopharmacy, University Hospital and KU Leuven
  • , Koen Van LaereAffiliated withDivision of Nuclear Medicine, University Hospital and KU LeuvenMolecular Small Animal Imaging Center (MoSAIC), KU Leuven

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Abstract

Purpose

Several lines of evidence strongly implicate a dysfunctional endocannabinoid system (ECS) in eating disorders. Using [18F]MK-9470 and small animal positron emission tomography (PET), we investigated for the first time cerebral changes in type 1 cannabinoid (CB1) receptor binding in vivo in the activity-based rat model of anorexia (ABA), in comparison to distinct motor- and food-related control conditions and in relation to gender and behavioural variables.

Methods

In total, experiments were conducted on 80 Wistar rats (23 male and 57 female). Male rats were assigned to the cross-sectional conditions: ABA (n = 12) and CONTROL (n = 11), whereas female rats were divided between two settings: (1) a cross-sectional design using ABA (n = 13), CONTROL (n = 9), and two extra control conditions for each of the variables manipulated in ABA, i.e. DIET (n = 8) and WHEEL (n = 9), and (2) a longitudinal one using ABA (n = 10) and CONTROL (n = 8) studied at baseline, during the model and upon recovery. The ABA group was subjected to food restriction in the presence of a running wheel, the DIET group to food restriction without wheel, the WHEEL group to a normal diet with wheel and CONTROL animals had a normal diet and no running wheel. Parametric CB1 receptor images of each group were spatially normalized to Paxinos space and analysed voxel-wise.

Results

In the ABA model, absolute [18F]MK-9470 binding was significantly increased in all cortical and subcortical brain areas as compared to control conditions (male +67 %; female >51 %, all p cluster < 6.3×10−6) that normalized towards baseline values after weight gain. Additionally, relative [18F]MK-9470 binding was increased in the hippocampus, inferior colliculus and entorhinal cortex of female ABA (+4.6 %; p cluster < 1.3×10−6), whereas no regional differences were observed in male subjects. Again, relative [18F]MK-9470 binding values normalized upon weight gain.

Conclusion

These data point to a widespread transient disturbance of the endocannabinoid transmission, specifically for CB1 receptors in the ABA model. Our data also suggest (1) gender effects on regional CB1 receptor binding in the hippocampus and (2) add further proof to the validity of the ABA model to mimic aspects of human disease.

Keywords

Type 1 cannabinoid receptor Anorexia nervosa ABA Small animal PET [18F]MK-9470