Applied Microbiology and Biotechnology

, Volume 101, Issue 1, pp 455–464

High throughput microencapsulation of Bacillus subtilis in semi-permeable biodegradable polymersomes for selenium remediation

  • Jacob Barlow
  • Kevin Gozzi
  • Chase P. Kelley
  • Benjamin M. Geilich
  • Thomas J. Webster
  • Yunrong Chai
  • Srinivas Sridhar
  • Anne L. van de Ven
Environmental biotechnology

DOI: 10.1007/s00253-016-7896-7

Cite this article as:
Barlow, J., Gozzi, K., Kelley, C.P. et al. Appl Microbiol Biotechnol (2017) 101: 455. doi:10.1007/s00253-016-7896-7

Abstract

Encapsulating bacteria within constrained microenvironments can promote the manifestation of specialized behaviors. Using double-emulsion droplet-generating microfluidic synthesis, live Bacillus subtilis bacteria were encapsulated in a semi-permeable membrane composed of poly(ethylene glycol)-b-poly(d,l-lactic acid) (mPEG-PDLLA). This polymer membrane was sufficiently permeable to permit exponential bacterial growth, metabolite-induced gene expression, and rapid biofilm growth. The biodegradable microparticles retained structural integrity for several days and could be successfully degraded with time or sustained bacterial activity. Microencapsulated B. subtilis successfully captured and contained sodium selenite added outside the polymersomes, converting the selenite into elemental selenium nanoparticles that were selectively retained inside the polymer membrane. This remediation of selenium using polymersomes has high potential for reducing the toxicity of environmental selenium contamination, as well as allowing selenium to be harvested from areas not amenable to conventional waste or water treatment.

Keywords

Microparticles Microfluidics Double-emulsion Bacteria Biofilm Selenite Nanoparticles 

Supplementary material

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Jacob Barlow
    • 1
  • Kevin Gozzi
    • 2
  • Chase P. Kelley
    • 1
  • Benjamin M. Geilich
    • 3
  • Thomas J. Webster
    • 1
    • 4
  • Yunrong Chai
    • 2
  • Srinivas Sridhar
    • 5
    • 6
  • Anne L. van de Ven
    • 5
    • 6
  1. 1.Department of Chemical EngineeringNortheastern UniversityBostonUSA
  2. 2.Department of BiologyNortheastern UniversityBostonUSA
  3. 3.Department of BioengineeringNortheastern UniversityBostonUSA
  4. 4.Center of Excellence for Advanced Materials ResearchKing Abdulaziz UniversityJeddahSaudi Arabia
  5. 5.Department of PhysicsNortheastern UniversityBostonUSA
  6. 6.Nanomedicine Science and Technology CenterNortheastern UniversityBostonUSA