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Nitrogen removal from synthetic wastewater using single and mixed culture systems of denitrifying fungi, bacteria, and actinobacteria

  • Environmental biotechnology
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Abstract

The aim of this study was to investigate the effects of single and mixed culture of denitrifying fungi, bacteria, and actinobacteria on nitrogen removal and N2O emission in treatment of wastewater. Denitrifying endophytes of Pseudomonas sp. B2, Streptomyces sp. A9, and Fusarium sp. F3 isolated from rice plants were utilized for treatment of synthetic wastewater containing nitrate and nitrite. Experiments were conducted under shaking and static conditions. Results showed that under the static condition, more than 97 % of nitrate removal efficiencies were reached in all the treatments containing B2. The nitrate removal rates within the first 12 h in the treatments of B2, B2+A9, B2+F3, and B2+A9+F3 were 7.3, 9.8, 11, and 11 mg L−1 h−1, respectively. Under the shaking condition, 100 % of nitrite was removed in all the treatments containing B2. The presence of A9 and F3 with B2 increased the nitrite removal rates under both the shaking and static conditions. Compared to the B2 system, the mixed systems of B2+A9, B2+F3, and B2+A9+F3 reduced N2O emission (78.4 vs. 19.4, 1.80, and 0.03 μM in 4 weeks, respectively). Our results suggested that B2 is an important strain that enhances nitrogen removal from wastewater. Mixed cultures of B2 with A9 and F3 can remove more nitrate and nitrite from wastewater and reduce nitrite accumulation and N2O emission in the denitrification process.

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Acknowledgments

This work was partly supported by a grant from the Chinese National Natural Science Foundation (No. 41471181).

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Authors and Affiliations

  1. School of Environmental Science and Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou, 510275, China

    Wenfeng Wang & Renduo Zhang

  2. School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China

    Lixiang Cao & Hongming Tan

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  1. Wenfeng Wang
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  2. Lixiang Cao
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  3. Hongming Tan
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  4. Renduo Zhang
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Correspondence to Lixiang Cao or Renduo Zhang.

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Wang, W., Cao, L., Tan, H. et al. Nitrogen removal from synthetic wastewater using single and mixed culture systems of denitrifying fungi, bacteria, and actinobacteria. Appl Microbiol Biotechnol 100, 9699–9707 (2016). https://doi.org/10.1007/s00253-016-7800-5

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