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Substrates and enzyme activities related to biotransformation of resveratrol from phenylalanine by Alternaria sp. MG1

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Abstract

To identify the substrates and enzymes related to resveratrol biosynthesis in Alternaria sp. MG1, different substrates were used to produce resveratrol, and their influence on resveratrol production was analyzed using high performance liquid chromatography (HPLC). Formation of resveratrol and related intermediates was identified using mass spectrum. During the biotransformation, activities of related enzymes, including phenylalanine ammonia-lyase (PAL), trans-cinnamate 4-hydroxylase (C4H), and 4-coumarate-CoA ligase (4CL), were analyzed and tracked. The reaction system contained 100 mL 0.2 mol/L phosphate buffer (pH 6.5), 120 g/L Alternaria sp. MG1 cells, 0.1 g/L MgSO4, and 0.2 g/L CaSO4 and different substrates according to the experimental design. The biotransformation was carried out for 21 h at 28 °C and 120 rpm. Resveratrol formation was identified when phenylalanine, tyrosine, cinnamic acid, and p-coumaric acid were separately used as the only substrate. Accumulation of cinnamic acid, p-coumaric acid, and resveratrol and the activities of PAL, C4H, and 4CL were identified and changed in different trends during transformation with phenylalanine as the only substrate. The addition of carbohydrates and the increase of phenylalanine concentration promoted resveratrol production and yielded the highest value (4.57 μg/L) when 2 g/L glucose, 1 g/L cyclodextrin, and phenylalanine (4.7 mmol/L) were used simultaneously.

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Acknowledgments

This work was supported by the Agriculture Department of China through Project no. CARS-30 and 201003021.

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Authors and Affiliations

  1. College of Food Science and Engineering, Northwest A&F University, 28 Xinong Road, Yangling, Shaanxi Province, 712100, China

    Jinhua Zhang & Junling Shi

  2. School of Life Sciences, Northwestern Polytechnical University, 127 Youyi Xilu, Xi’an, Shaanxi Province, 710072, China

    Junling Shi

  3. College of Enology, Northwest A&F University, 23 Xinong Road, Yangling, Shaanxi Province, 712100, China

    Yanlin Liu

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  1. Jinhua Zhang
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Correspondence to Junling Shi.

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Zhang, J., Shi, J. & Liu, Y. Substrates and enzyme activities related to biotransformation of resveratrol from phenylalanine by Alternaria sp. MG1. Appl Microbiol Biotechnol 97, 9941–9954 (2013). https://doi.org/10.1007/s00253-013-5212-3

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