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Bioremediation of the tobacco waste-contaminated soil by Pseudomonas sp. HF-1: nicotine degradation and microbial community analysis

  • Environmental biotechnology
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Abstract

The highly effective nicotine-degrading bacterium Pseudomonas sp. HF-1 was augmented into the tobacco waste-contaminated soil to degrade nicotine and evaluate the effect of the bioremediation. Comparing with non-adding (NA) systems, the treatments with addition of strain HF-1 (TA) exhibited considerably stronger pollution disposal abilities and higher stability of pH value and moisture content, especially in groups containing a large quantity of tobacco waste. The denaturing gradient gel electrophoresis (DGGE) profiles showed that the Shannon–Wiener index decreased with increasing wastes in the NA treatments, while a gradual increase was found in the TA groups. A comparison of sequences from DGGE bands demonstrated that there were differences in the dominant microbial species between the two treatments, suggesting that strain HF-1 could persist in the soil and enhance the efficiency of tobacco waste disposal. The results of real-time fluorescence quantitative PCR (RT-qPCR) also indicated that strain HF-1 existed in the TA systems and grew with relative high quantities. In conclusion, the nicotine-degrading strain HF-1 played a leading role in the bioremediation of the tobacco waste-contaminated soil and influenced the dynamics and structure of the microbial community.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (no. 31170115, 31100032) and the Major Science and Technology Program for Water Pollution Control and Treatment (no. 2012ZX07101-012). We are sincerely grateful to Mr. Philip Alexzander Lorhrmann for greatly improving the use of English.

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Authors and Affiliations

  1. Institute of Microbiology, College of Life Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China

    Xin Wang, Lu Tang, Haixia Wang, Hang Min & Zhenmei Lu

  2. Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, Hangzhou, 310021, China

    Yanlai Yao

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  1. Xin Wang
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  2. Lu Tang
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  3. Yanlai Yao
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Correspondence to Zhenmei Lu.

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Wang, X., Tang, L., Yao, Y. et al. Bioremediation of the tobacco waste-contaminated soil by Pseudomonas sp. HF-1: nicotine degradation and microbial community analysis. Appl Microbiol Biotechnol 97, 6077–6088 (2013). https://doi.org/10.1007/s00253-012-4433-1

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