Applied Microbiology and Biotechnology

, Volume 97, Issue 13, pp 6077–6088

Bioremediation of the tobacco waste-contaminated soil by Pseudomonas sp. HF-1: nicotine degradation and microbial community analysis

  • Xin Wang
  • Lu Tang
  • Yanlai Yao
  • Haixia Wang
  • Hang Min
  • Zhenmei Lu
Environmental biotechnology

DOI: 10.1007/s00253-012-4433-1

Cite this article as:
Wang, X., Tang, L., Yao, Y. et al. Appl Microbiol Biotechnol (2013) 97: 6077. doi:10.1007/s00253-012-4433-1

Abstract

The highly effective nicotine-degrading bacterium Pseudomonas sp. HF-1 was augmented into the tobacco waste-contaminated soil to degrade nicotine and evaluate the effect of the bioremediation. Comparing with non-adding (NA) systems, the treatments with addition of strain HF-1 (TA) exhibited considerably stronger pollution disposal abilities and higher stability of pH value and moisture content, especially in groups containing a large quantity of tobacco waste. The denaturing gradient gel electrophoresis (DGGE) profiles showed that the Shannon–Wiener index decreased with increasing wastes in the NA treatments, while a gradual increase was found in the TA groups. A comparison of sequences from DGGE bands demonstrated that there were differences in the dominant microbial species between the two treatments, suggesting that strain HF-1 could persist in the soil and enhance the efficiency of tobacco waste disposal. The results of real-time fluorescence quantitative PCR (RT-qPCR) also indicated that strain HF-1 existed in the TA systems and grew with relative high quantities. In conclusion, the nicotine-degrading strain HF-1 played a leading role in the bioremediation of the tobacco waste-contaminated soil and influenced the dynamics and structure of the microbial community.

Keywords

Pseudomonas sp. HF-1Tobacco waste-contaminated soilBioremediationMicrobial communityReal-time qPCR

Supplementary material

253_2012_4433_MOESM1_ESM.pdf (545 kb)
ESM 1(PDF 544 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Xin Wang
    • 1
  • Lu Tang
    • 1
  • Yanlai Yao
    • 2
  • Haixia Wang
    • 1
  • Hang Min
    • 1
  • Zhenmei Lu
    • 1
  1. 1.Institute of Microbiology, College of Life ScienceZhejiang UniversityHangzhouChina
  2. 2.Institute of Environment, Resource, Soil and FertilizerZhejiang Academy of Agricultural SciencesHangzhouChina