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Bioconversion of p-coumaric acid to p-hydroxystyrene using phenolic acid decarboxylase from B. amyloliquefaciens in biphasic reaction system

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Abstract

Phenolic acid decarboxylase (PAD) catalyzes the non-oxidative decarboxylation of p-coumaric acid (pCA) to p-hydroxystyrene (pHS). PAD from Bacillus amyloliquefaciens (BAPAD), which showed k cat/K m value for pCA (9.3 × 103 mM−1 s−1), was found as the most active one using the “Subgrouping Automata” program and by comparing enzyme activity. However, the production of pHS of recombinant Escherichia coli harboring BAPAD showed only a 22.7 % conversion yield due to product inhibition. Based on the partition coefficient of pHS and biocompatibility of the cell, 1-octanol was selected for the biphasic reaction. The conversion yield increased up to 98.0 % and 0.83 g/h/g DCW productivity was achieved at 100 mM pCA using equal volume of 1-octanol as an organic solvent. In the optimized biphasic reactor, using a three volume ratio of 1-octanol to phosphate buffer phase (50 mM, pH 7.0), the recombinant E. coli produced pHS with a 88.7 % conversion yield and 1.34 g/h/g DCW productivity at 300 mM pCA.

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Acknowledgments

This research was supported by World Class University program through the National Research Foundation of Korea grant funded by the Ministry of Education, Science and Technology (R322009000102130).

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Authors and Affiliations

  1. School of Chemical and Biological Engineering, Seoul National University, Seoul, #151-742, Republic of Korea

    Da-Hye Jung, Wonji Choi, Kwon-Young Choi, Eunok Jung, Romas J. Kazlauskas & Byung-Gee Kim

  2. School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, #712-749, Republic of Korea

    Hyungdon Yun

  3. Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 1479 Gortner Avenue, 174 Gortner Lab, St. Paul, MN, 55108, USA

    Romas J. Kazlauskas

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  1. Da-Hye Jung
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  2. Wonji Choi
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  3. Kwon-Young Choi
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  4. Eunok Jung
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Correspondence to Byung-Gee Kim.

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Jung, DH., Choi, W., Choi, KY. et al. Bioconversion of p-coumaric acid to p-hydroxystyrene using phenolic acid decarboxylase from B. amyloliquefaciens in biphasic reaction system. Appl Microbiol Biotechnol 97, 1501–1511 (2013). https://doi.org/10.1007/s00253-012-4358-8

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