Abstract
Guanosine 5′-triphosphate (GTP) is the key substrate for biosynthesis of guanosine 5′-diphosphate (GDP)-l-fucose. In this study, improvement of GDP-l-fucose production was attempted by manipulating the biosynthetic pathway for guanosine nucleotides in recombinant Escherichia coli-producing GDP-l-fucose. The effects of overexpression of inosine 5′-monophosphate (IMP) dehydrogenase, guanosine 5′-monophosphate (GMP) synthetase (GuaB and GuaA), GMP reductase (GuaC) and guanosine–inosine kinase (Gsk) on GDP-l-fucose production were investigated in a series of fed-batch fermentations. Among the enzymes tested, overexpression of Gsk led to a significant improvement of GDP-l-fucose production. Maximum GDP-l-fucose concentration of 305.5 ± 5.3 mg l−1 was obtained in the pH-stat fed-batch fermentation of recombinant E. coli-overexpressing Gsk, which corresponds to a 58% enhancement in the GDP-l-fucose production compared with the control strain overexpressing GDP-l-fucose biosynthetic enzymes. Such an enhancement of GDP-l-fucose production could be due to the increase in the intracellular level of GMP.
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Acknowledgement
This work was supported by a Korea Research Council of Fundamental Science and Technology (KRCF) grant. This work was also supported by the Advenced Biomass R&D Center (ABC) of Korea Grant funded by the Ministry of Education, Science and Technology (2010–0029799). MD Kim was supported by the Regional Technology Innovation Program of the Ministry of Knowledge Economy (RTI05-01-02).
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Lee, WH., Shin, SY., Kim, MD. et al. Modulation of guanosine nucleotides biosynthetic pathways enhanced GDP-l-fucose production in recombinant Escherichia coli . Appl Microbiol Biotechnol 93, 2327–2334 (2012). https://doi.org/10.1007/s00253-011-3776-3
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DOI: https://doi.org/10.1007/s00253-011-3776-3