Applied Microbiology and Biotechnology

, Volume 89, Issue 5, pp 1611–1619

Growth and polyhydroxybutyrate production by Ralstonia eutropha in emulsified plant oil medium


  • Charles F. Budde
    • Department of Chemical EngineeringMassachusetts Institute of Technology
  • Sebastian L. Riedel
    • Department of BiologyMassachusetts Institute of Technology
  • Florian Hübner
    • Department of BiotechnologyBeuth Hochschule für Technik Berlin
  • Stefan Risch
    • Department of BiotechnologyBeuth Hochschule für Technik Berlin
  • Milan K. Popović
    • Department of BiotechnologyBeuth Hochschule für Technik Berlin
  • ChoKyun Rha
    • Biomaterials Science and Engineering LaboratoryMassachusetts Institute of Technology
    • Department of BiologyMassachusetts Institute of Technology
    • Division of Health Sciences and TechnologyMassachusetts Institute of Technology
Applied Microbial and Cell Physiology

DOI: 10.1007/s00253-011-3102-0

Cite this article as:
Budde, C.F., Riedel, S.L., Hübner, F. et al. Appl Microbiol Biotechnol (2011) 89: 1611. doi:10.1007/s00253-011-3102-0


Polyhydroxyalkanoates (PHAs) are natural polyesters synthesized by bacteria for carbon and energy storage that also have commercial potential as bioplastics. One promising class of carbon feedstocks for industrial PHA production is plant oils, due to the high carbon content of these compounds. The bacterium Ralstonia eutropha accumulates high levels of PHA and can effectively utilize plant oil. Growth experiments that include plant oil, however, are difficult to conduct in a quantitative and reproducible manner due to the heterogeneity of the two-phase medium. In order to overcome this obstacle, a new culture method was developed in which palm oil was emulsified in growth medium using the glycoprotein gum arabic as the emulsifying agent. Gum arabic did not influence R. eutropha growth and could not be used as a nutrient source by the bacteria. R. eutropha was grown in the emulsified oil medium and PHA production was measured over time. Additionally, an extraction method was developed to monitor oil consumption. The new method described in this study allows quantitative, reproducible R. eutropha experiments to be performed with plant oils. The method may also prove useful for studying growth of different bacteria on plant oils and other hydrophobic carbon sources.


Ralstonia eutrophaPolyhydroxyalkanoatePolyhydroxybutyratePlant oilPalm oil

Copyright information

© Springer-Verlag 2011