Mini-Review

Applied Microbiology and Biotechnology

, Volume 88, Issue 6, pp 1233-1242

Open Access This content is freely available online to anyone, anywhere at any time.

Engineered polyketide biosynthesis and biocatalysis in Escherichia coli

  • Xue GaoAffiliated withDepartment of Chemical and Biomolecular Engineering, University of California
  • , Peng WangAffiliated withDepartment of Chemical and Biomolecular Engineering, University of California
  • , Yi TangAffiliated withDepartment of Chemical and Biomolecular Engineering, University of CaliforniaDepartment of Chemistry and Biochemistry, University of California Email author 

Abstract

Polyketides are important bioactive natural products biosynthesized by bacteria, fungi, and plants. The enzymes that synthesize polyketides are collectively referred to as polyketide synthases (PKSs). Because many of the natural hosts that produce polyketides are difficult to culture or manipulate, establishing a universal heterologous host that is genetically tractable has become an important goal toward the engineered biosynthesis of polyketides and analogues. Here, we summarize the recent progresses in engineering Escherichia coli as a heterologous host for reconstituting PKSs of different types. Our increased understanding of PKS enzymology and structural biology, combined with new tools in protein engineering, metabolic engineering, and synthetic biology, has firmly established E. coli as a powerful host for producing polyketides.

Keyword

Biosynthesis Megasynthase Heterologous host