Environmental Biotechnology

Applied Microbiology and Biotechnology

, Volume 88, Issue 6, pp 1413-1422

Response of microbial community structure to microbial plugging in a mesothermic petroleum reservoir in China

  • Fan ZhangAffiliated withThe Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, Ministry of Education, School of Energy Resources, China University of Geosciences (Beijing) Email author 
  • , Yue Hui SheAffiliated withCollege of Chemistry and Molecular Sciences, Wuhan UniversityCollege of Chemistry and Environmental Engineering, Yangtze University Email author 
  • , Sha Sha MaAffiliated withCollege of Chemistry and Environmental Engineering, Yangtze University
  • , Ji Ming HuAffiliated withCollege of Chemistry and Molecular Sciences, Wuhan University
  • , Ibrahim M. BanatAffiliated withSchool of Biomedical Sciences, University of Ulster
  • , Du Jie HouAffiliated withThe Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, Ministry of Education, School of Energy Resources, China University of Geosciences (Beijing) Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Microbial plugging, a microbial enhancement of oil recovery (MEOR) technique, has been applied in a candidate oil reservoir of Daqing Oil Field (China). The goal of this study is to monitor the survival of injected bacteria and reveal the response of microbial communities in field trial of microbial plugging through injection of selected microbial culture broth and nutrients. Culture-dependent enrichment and culture-independent 16S rDNA clone library methods were used. The results show that it was easy to activate targeted biopolymer-producing bacteria in a laboratory environment, and it was difficult for injected exogenous bacteria to survive. In addition, microbial communities in the oil reservoir also changed before and after the field trial. However, microbial communities, activated by fermentative medium for biopolymer-producing bacteria, appeared to show greater differences in the laboratory than in the natural reservoir. It was concluded that microbial populations monitoring was important to MEOR; results of response of microbial communities could provide a guide for the future field trials.

Keywords

Microbial plugging Microbial enhancement of oil recovery (MEOR) Microbial communities Biopolymer-producing bacteria 16S rDNA clone library