Methods and Protocols

Applied Microbiology and Biotechnology

, Volume 87, Issue 5, pp 1917-1925

Antibacterial activity of ZnO nanoparticles prepared via non-hydrolytic solution route

  • Rizwan WahabAffiliated withEnergy Materials and Surface Science Laboratory, Solar Energy Research Center, School of Chemical Engineering, Chonbuk National University
  • , Amrita MishraAffiliated withDepartment of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University
  • , Soon-Il YunAffiliated withDepartment of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University
  • , Young-Soon KimAffiliated withEnergy Materials and Surface Science Laboratory, Solar Energy Research Center, School of Chemical Engineering, Chonbuk National University
  • , Hyung-Shik ShinAffiliated withEnergy Materials and Surface Science Laboratory, Solar Energy Research Center, School of Chemical Engineering, Chonbuk National University Email author 

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Abstract

The antibacterial activity of ZnO nanoparticles has been investigated and presented in this paper. Nanoparticles were prepared via non-hydrolytic solution process using zinc acetate di-hydrate (Zn(CH3COO)2·2H2O) and aniline (C6H5NH2) in 6 h refluxing at ∼65 °C. In the presence of four pathogens such as Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Klebsiella pneumoniae, the antibacterial study of zinc oxide nanoparticles were observed. The antibacterial activity of ZnO nanoparticles (ZnO-NPs) were studied by spectroscopic method taking different concentrations (5–45 μg/ml) of ZnO-NPs. Our investigation reveals that the lowest concentration of ZnO-NPs solution inhibiting the growth of microbial strain is found to be 5 μg/ml for K. pneumoniae, whereas for E. coli, S. aureus, and S. typhimurium, it was calculated to be 15 μg/ml. The diameter of each ZnO-NPs lies between “20 and 30 nm” as observed from FESEM and transmission electron microscopy images. The composition of synthesized material was analyzed by the Fourier transform infrared spectroscopy, and it shows the band of ZnO at 441 cm−1. Additionally, on the basis of morphological and chemical observations, the chemical reaction mechanism of ZnO-NPs was also proposed.

Keywords

Pathogenic bacteria X-ray diffraction pattern (XRD) PL spectroscopy FTIR spectroscopy ZnO nanoparticles and antibacterial activity