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Chemical characterization, antiproliferative and antiadhesive properties of polysaccharides extracted from Pleurotus pulmonarius mycelium and fruiting bodies

  • Applied Microbial and Cell Physiology
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Abstract

Mushroom polysaccharides are potent substances that exhibit antitumor and immunomodulatory properties. Studies comparing the chemical composition and antitumor-related activities of polysaccharides released by fungal strains under different growth conditions are not available. Thus, the present study compared polysaccharides extracts produced by Pleurotus pulmonarius from mycelium grown in liquid culture (ME) or fruiting bodies (FBE). Polysaccharides of both ME and FBE had a relatively high molecular mass. NMR spectroscopy indicated that ME glucan is an α-glucan whereas FBE glucan is a mixture of both α- and β-glucans. Glucose and galactose where the most prominent monosaccharide in both glucans. Treatment of several colon cancer cell lines expressing varying amounts of galectin-3 with the two fungal glucans inhibited their viability and significantly reduced their ability to adhere to the key component of the extracellular matrix, fibronectin, and to a human umbilical vein endothelial cell monolayer, in a time- and dose-dependent manner mainly in those cell lines expressing high amounts of galectin-3. We conclude that ME and FBE glucans may exert a direct antiproliferative effect on cancer cells expressing high galectin-3 concentrations and concomitantly downregulate tumor cell adherence, the latter being directly related to cancer progression and metastasis.

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Authors and Affiliations

  1. Institute of Biochemistry, Food Science and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel

    Iris Lavi, Irena Peri & Betty Schwartz

  2. Department of Plant Pathology and Microbiology, The Hebrew University of Jerusalem, Rehovot, Israel

    Dana Levinson & Yitzhak Hadar

  3. Department of Biotechnology, Ben-Gurion University of the Negev, Beersheba, Israel

    Yoram Tekoah

Authors
  1. Iris Lavi
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  2. Dana Levinson
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  3. Irena Peri
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  4. Yoram Tekoah
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  5. Yitzhak Hadar
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  6. Betty Schwartz
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Correspondence to Betty Schwartz.

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This study was partially supported by a Grant from the “Yissum Research Development Company of the Hebrew University of Jerusalem.”

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Lavi, I., Levinson, D., Peri, I. et al. Chemical characterization, antiproliferative and antiadhesive properties of polysaccharides extracted from Pleurotus pulmonarius mycelium and fruiting bodies. Appl Microbiol Biotechnol 85, 1977–1990 (2010). https://doi.org/10.1007/s00253-009-2296-x

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  • DOI: https://doi.org/10.1007/s00253-009-2296-x

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