Applied Microbiology and Biotechnology

, Volume 75, Issue 3, pp 655–663

Polycyclic aromatic hydrocarbon-degrading Mycobacterium isolates: their association with plant roots

Authors

  • R. Child
    • Department of BiologyUtah State University
  • C. D. Miller
    • Department of BiologyUtah State University
  • Y. Liang
    • Department of Biological Engineering and IrrigationUtah State University
  • G. Narasimham
    • Department of Biological Engineering and IrrigationUtah State University
  • J. Chatterton
    • USDA Forage and Range Research LaboratoryUtah State University
  • P. Harrison
    • USDA Forage and Range Research LaboratoryUtah State University
  • R. C. Sims
    • Department of Biological Engineering and IrrigationUtah State University
  • D. Britt
    • Department of Biological Engineering and IrrigationUtah State University
    • Department of BiologyUtah State University
Environmental Biotechnology

DOI: 10.1007/s00253-007-0840-0

Cite this article as:
Child, R., Miller, C.D., Liang, Y. et al. Appl Microbiol Biotechnol (2007) 75: 655. doi:10.1007/s00253-007-0840-0

Abstract

Five environmental mycobacterium isolates that degrade polycyclic aromatic hydrocarbons (PAHs) were associated with barley root surfaces after growth of the seedlings from inoculated seed. Mycobacterium cells were detected along the total root length for four of these isolates. These PAH-degrading mycobacterium strains had hydrophilic cell surfaces, whereas one strain, MCS, that was hydrophobic had reduced association along the root length with no cells being detected from the root tips. The root-tip-competent strain, KMS, was competitive for its root association in the presence of the root-colonizing pseudomonad, Pseudomonas putida KT2440. All mycobacterium strains utilized simple sugars (fructose, glucose) and the trisaccharide 6-kestose, present in barley root washes, for planktonic growth, but they differed in their potential for biofilm formation under in vitro conditions. Mineralization of pyrene by the KMS strain occurred when the components in the barley root wash were amended with labeled pyrene suggesting to us that mineralization could occur in plant rhizospheres containing such mycobacterium strains.

Keywords

Polycyclic aromatic hydrocarbonsRoot colonizationMycobacteriumRemediation

Copyright information

© Springer-Verlag 2007