Abstract
Clostridial acetone–butanol fermentation from renewable carbohydrates used to be the largest biotechnological process second only to yeast ethanol fermentation and the largest process ever run under sterile conditions. With the rising prices for mineral oil, it has now the economical and technological potential to replace petrochemistry for the production of fuels from renewable resources. Various methods for using non-food biomass such as cellulose and hemicellulose in agricultural products and wastes have been developed at laboratory scale. To our knowledge, the AB plants in Russia were the only full-scale industrial plants which used hydrolyzates of lignocellosic waste for butanol fermentation. These plants were further developed into the 1980s, and the process was finally run in a continual mode different from plants in Western countries. A biorefinery concept for the use of all by-products has been elaborated and was partially put into practice. The experience gained in the Soviet Union forms a promising basis for the development of modern large-scale processes to replace a considerable fraction of the current chemical production of fuel for our future needs on a sustainable basis.
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References
Andrade JC, Vasconcelos I (2003) Continuous cultures of Clostridium acetobutylicum: culture stability and low-grade glycerol utilisation. Biotechnol Lett 25:121–125
Andreesen JR, Gottschalk G (1969) The occurrence of a modified Entner–Doudoroff pathway in Clostridium aceticum. Arch Mikrobiol 69:160–170
Awang GM, Jones GA, Ingledew WM (1988) The acetone–butanol–ethanol fermentation. Crit Rev Microbiol 15(Suppl 1):33–67
Bahl H, Andersch W, Gottschalk G (1982) Continuous production of acetone and butanol by Clostridium acetobutylicum in a two-stage phosphate limited chemostat. Eur J Appl Microbiol Biotechnol 15:201–205
Claassen PAM, van Lier JB, Lopez-Contreras AM, van Niel EWJ, Sijtsma L, Stams AJM, de Vries SS, Weutshuis RA (1999) Utilisation of biomass for the supply of energy carriers. Appl Microbiol Biotechnol 52:741–755
Collins MD, Lawson PA, Willems A, Cordoba JJ, Fernandez-Garayzabal J, Garcia P, Cai J, Hippe H, Farrow JAE (1994) The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826
Cornillot E, Nair RV, Papoutsakis ET, Soucaille P (1997) The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC824 reside on a large plasmid whose loss leads to degeneration of the strain. J Bacteriol 179:5442–5447
Dale BE (1987) Lignocellulose conversion and the future of fermentation biotechnology. Trends Biotechnol 5:287–291
Demain AL, Newcomb M, Wu JHD (2005) Cellulase, clostridia, and ethanol. Microbiol Mol Biol Rev 69:124–154
Dürre P (1998) New insights and novel developments in clostridial acetone/butanol/ isopropanol fermentation. Appl Microbiol Biotechnol 49:639–648
Dürre P, Bohringer M, Nakotte S, Schaffer S, Thormann K, Zickner B (2002) Transcriptional regulation of solventogenesis in Clostridium acetobutylicum. J Mol Microbiol Biotechnol 4:295–300
Ezeji TC, Groberg M, Qureshi N, Blaschek HP (2003) Continuous production of butanol from starch-based packing peanuts. Appl Biochem Biotechnol 108:375–382
Ezeji TC, Karcher PM, Qureshi N, Blaschek HP (2005) Improving performance of a gas stripping-based recovery system to remove butanol from Clostridium beijerinckii fermentation. Bioprocess Biosyst Eng 27:207–214
Galbe M, Zacchi G (2002) A review of the production of ethanol from softwood. Appl Microbiol Biotechnol 59:618–628
Gapes JR (2000a) The economics of acetone–butanol fermentation: theoretical and market considerations. J Mol Microbiol Biotechnol 2:27–32
Gapes JR (2000b) The history of the acetone–butanol project in Austria. J Mol Microbiol Biotechnol 2:5–8
Girbal L, Soucaille P (1994) Regulation of Clostridium acetobutylicum metabolism as revealed by mixed-substrate steady-state continuous cultures: role of NADH/NAD ratio and ATP pool. J Bacteriol 176:6433–6438
Gottschal JC, Morris JG (1982) Continuous production of acetone and butanol by Clostridium acetobutylicaum growing in turbidostat culture. Biotechnol Lett 4:477–482
Greene N, Celik FE, Dale B, Jackson M, Jayawardhana K, Jin H, Larson ED, Laser M, Lynd L, MacKenzie D, Mark J, McBride J, McLaughlin S, Saccardi D (2004) Growing energy. How biofuels can help end America’s oil dependence. NRDC, USA
Groot et al (1992) Integration of pervaporation and continuous butanol fermentation with immobilized cells. Chem Eng J 46:B1–B10
Haggstrom L (1985) Acetone–butanol fermentation and its variants. Biotechnol Adv 3:13–28
Hazlewood GP, Gilbert HJ (1993) Xylan and cellulose utilization by the clostridia. Biotechnology 25:311–341
Huang WC, Ramey DE, Yang ST (2004) Continuous production of butanol by Clostridium acetobutylicum immobilized in a fibrous bed bioreactor. Appl Biochem Biotechnol 113–116:887–898
Iarovenko VL, Nakhmanovich BM, Shcheblykin NP, Senkevich VV (1960) A study of continuous acetone butylic fermentation caused by Clostridium acetobutylicum. Mikrobiologiia 29:581–586
Jones DT, Keis S (1995) Origins and relationships of industrial solvent-producing clostridial strains. FEMS Microbiol Rev 17:223–232
Jones DT, Woods DR (1986) Acetone–butanol fermentation revisited. Microbiol Rev 50:484–524
Kashket ER, Cao ZY (1995) Clostridial strain degeneration. FEMS Microbiol Rev 17:307–315
Keis S, Bennett CF, Ward VK, Jones DT (1995) Taxonomy and phylogeny of industrial solvent-producing bacteria. Int J Syst Bacteriol 45:693–705
Keis S, Shaheen R, Jones DT (2001) Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp. nov. and Clostridium saccharobutylicum sp. nov. Int J Syst Evol Microbiol 51:2095–2103
Langlykke AF, van Lanen JM, Fraser DR (1948) Butyl alcohol from xylose saccharification liquors from corn-cobs. Ind Eng Chem 40:1716–1719
Logotkin IS (1958) Technology of acetone–butanol production (in Russian). Pisshprom Isdat, Moscow
Lopez-Contreras AM, Gabor K, Martens AA, Renckens BA, Claassen PA, Van Der Oost J, De Vos WM (2004) Substrate-induced production and secretion of cellulases by Clostridium acetobutylicum. Appl Environ Microbiol 70:5238–5243
Lynd LR, Cushman JH, Nichols RJ, Wyman CE (1991) Fuel ethanol from cellulosic biomass. Science 251:1318–1322
Lynd LR, Wyman CE, Gerngross TU (1999) Biocommodity engineering. Biotechnol Prog 15:777–793
Lynd LR, Zyl WH, McBride JE, Laser M (2005) Consolidated bioprocessing of cellulosic biomass: an update. Curr Opin Biotechnol 16:577–583
Maddox IS, Steiner E, Hirsch S, Wessner S, Gutierrez NA, Gapes JR, Schuster KC (2000) The cause of “acid crash” and “acidogenic fermentations” during the batch acetone–butanol–ethanol (AB-) fermentation process. J Mol Microbiol Biotechnol 2:95–100
Montoya D, Arévalo C, Gonzales S, Aristizabal F, Schwarz WH (2001) New solvent-producing Clostridium sp. strains, hydrolyzing a wide range of polysaccharides, are closely related to Clostridium butyricum. J Ind Microbiol Biotechnol 27:329–335
Mutschlechner O, Swoboda H, Gapes JR (2000) Continuous two-stage AB-fermentation using Clostridium beijerinckii NRRL B592 operating with a growth rate in the first stage vessel close to its maximal value. J Mol Microbiol Biotechnol 2:101–105
Nakhmanovich BM, Shcheblykina NA (1959) Fermentation of pentoses of corn cob hydrolyzates by Clostridium acetobutylicum. Mikrobiologiya 28:99–104
Nativel F, Pourquie J, Ballerini D, Vandecasteele JP, Renault P (1992) The biotechnology facilities at Soustons for biomass conversion. Int J Sol Energy 11:219–229
Nimcevic D, Gapes JR (2000) The acetone–butanol fermentation in pilot plant and pre-industrial scale. J Mol Microbiol Biotechnol 2:15–20
Nölling J et al (2001) Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum. J Bacteriol 183:4823–4838
Riehm T (1962) Holzzucker und Holzzuckerlösungen. In: Die Hefen, vol II. Carl Hanser Verlag, Nürnberg, pp 82–113
Sabathe F, Soucaille P (2003) Characterization of the CipA scaffolding protein and in vivo production of a minicellulosome in Clostridium acetobutylicum. J Bacteriol 185:1092–1096
Sabathe F, Belaich A, Soucaille P (2002) Characterization of the cellulolytic complex (cellulosome) of Clostridium acetobutylicum. FEMS Microbiol Lett 217:15–22
Schuster KC, Goodacre R, Gapes JR, Young M (2001) Degeneration of solventogenic Clostridium strains monitored by Fourier transform infrared spectroscopy of bacterial cells. J Ind Microbiol Biotechnol 27:314–321
Schwarz WH (2001) The cellulosome and cellulose degradation by anaerobic bacteria. Appl Microbiol Biotechnol 56:634–649
Schwarz WH, Gapes R (2006) Butanol—rediscovering a renewable fuel. BioWorld Europe 1:16–19
Shaheen R, Shirley M, Jones DT (2000) Comparative fermentation studies of industrial strains belonging to four species of solvent-producing clostridia. J Mol Microbiol Biotechnol 2:115–124
Stout BA (1982) Conversion of biomass to fuel and chemical raw material. Experientia 38:145–151
Terracciano JS, Kashket ER (1986) Intracellular conditions required for initiation of solvent production by Clostridium acetobutylicum. Appl Environ Microbiol 52:86–91
Thomas SM, DiCosimo R, Nagarajan V (2002) Biocatalysts: applications and potentials for the chemical industry. Trends Biotechnol 20:238–242
Waksman S, Kirsh D (1933) Butyric acids and butyl alcohol fermentation of hemi-cellulose and starch-rich materials. Ind Eng Chem 25:1036–1041
Wilkinson SR, Young M (1993) Wide diversity of genome size among different strains of Clostridium acetobutylicum. J Gen Microbiol 139:1069–1076
Wilkinson SR, Young DI, Morris JG, Young M (1995) Molecular genetics and the initiation of solventogenesis in Clostridium beijerinckii (formerly Clostridium acetobutylicum) NCIMB 8052. FEMS Microbiol Rev 17:275–285
Wyman CE (2001) Twenty years of trials, tribulations, and research progress in bioethanol technology: selected key events along the way. Appl Biochem Biotechnol 91:5–21
Yarovenko VL, Nachmanovich BM, Shcheblykin NP (1962) Two-directional flow scheme of continuous acetone–butanol fermentation. Spiritovaya Promishlinost 1:11–15, (in Russian)
Yarovenko VL, Nachmanovich BM, Shcheblykin NP, Senkevich VV (1963) Continuous fermentation in acetone–butanol production. Kabardino-Balkariya Publishers, Nalchik (in Russian)
Yu EKC, Deschatelet L, Saddler JN (1984) The bioconversion of wood hydrolyzates to butanol and butanediol. Biotechnol Lett 6:327–332
Acknowledgements
The authors thank D. Antoni, H. Bahl, R. Gapes, R. Igelspacher, D. Jones, E. Kashket, J. Puls, D. Schieder, W. L. Staudenbauer, and S. Yarotsky for numerous comments, corrections, suggestions, and critically reading the manuscript. The support of an INTAS-YSF fellowship to OB is gratefully acknowledged.
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Zverlov, V.V., Berezina, O., Velikodvorskaya, G.A. et al. Bacterial acetone and butanol production by industrial fermentation in the Soviet Union: use of hydrolyzed agricultural waste for biorefinery. Appl Microbiol Biotechnol 71, 587–597 (2006). https://doi.org/10.1007/s00253-006-0445-z
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DOI: https://doi.org/10.1007/s00253-006-0445-z