Applied Microbiology and Biotechnology

, Volume 61, Issue 3, pp 189-196

First online:

Aspergillus niger citric acid accumulation: do we understand this well working black box?

  • Levente KaraffaAffiliated withDepartment of Microbiology and Biotechnology, Faculty of Sciences, University of Debrecen
  • , Christian P. KubicekAffiliated withDivision of Applied Biochemistry and Gene Technology, Institute of Chemical Engineering, TU Wien Email author 

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This Mini-Review summarizes the current knowledge on the biochemical and physiological events leading to massive citric acid accumulation by Aspergillus niger under industrially comparable conditions, thereby particularly emphasizing the roles of glycolytic flux and its control, excretion of citric acid from the mitochondria and the cytosol, and the critical fermentation variables. The potential of novel techniques for metabolic analysis and genomic approaches in understanding this fermentation is also discussed.