Original Paper

Immunogenetics

, Volume 52, Issue 3, pp 284-288

First online:

Localization of the Rh50-like protein to the contractile vacuole in Dictyostelium

  • Mohammed BenghezalAffiliated withUniversité de Genève, Centre Médical Universitaire, Département de Morphologie, 1 rue Michel Servet, 1211 Genève 4, Switzerland
  • , Daniel GotthardtAffiliated withDepartment of Molecular Cell Research, Max-Planck-Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
  • , Sophie CornillonAffiliated withUniversité de Genève, Centre Médical Universitaire, Département de Morphologie, 1 rue Michel Servet, 1211 Genève 4, Switzerland
  • , Pierre CossonAffiliated withUniversité de Genève, Centre Médical Universitaire, Département de Morphologie, 1 rue Michel Servet, 1211 Genève 4, Switzerland

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Abstract.

The human Rhesus (Rh) family consists of three polytopic membrane proteins present at the cell surface of red blood cells. Although Rh proteins are essential for the expression of the blood group system their biological function remains unclear. In this study, the gene encoding a protein homologous to Rh50 in Dictyostelium discoideum was sequenced. The Rh50-like protein was localized to the contractile vacuole, the organelle responsible for maintenance of osmotic equilibrium within the cell. However, Rh50-like-deficient mutants in which the Rh50-like gene was disrupted did not appear to exhibit a phenotype related to osmoregulation. Nevertheless, these mutants may provide a valuable tool for studying the function of the rhesus protein.

Dictyostelium Gene inactivation Immunolocalization Contractile vacuole Rhesus