Immunogenetics

, Volume 68, Issue 6, pp 417–428

Cynomolgus macaque (Macaca fascicularis) immunoglobulin heavy chain locus description

  • Guo-Yun Yu
  • Suzanne Mate
  • Karla Garcia
  • Michael D. Ward
  • Ernst Brueggemann
  • Matthew Hall
  • Tara Kenny
  • Mariano Sanchez-Lockhart
  • Marie-Paule Lefranc
  • Gustavo Palacios
Original Article

DOI: 10.1007/s00251-016-0921-2

Cite this article as:
Yu, GY., Mate, S., Garcia, K. et al. Immunogenetics (2016) 68: 417. doi:10.1007/s00251-016-0921-2

Abstract

Cynomolgus macaques (Macaca fascicularis) have become an important animal model for biomedical research. In particular, it is the animal model of choice for the development of vaccine candidates associated with emerging dangerous pathogens. Despite their increasing importance as animal models, the cynomolgus macaque genome is not fully characterized, hindering molecular studies for this model. More importantly, the lack of knowledge about the immunoglobulin (IG) locus organization directly impacts the analysis of the humoral response in cynomolgus macaques. Recent advances in next generation sequencing (NGS) technologies to analyze IG repertoires open the opportunity to deeply characterize the humoral immune response. However, the IG locus organization for the animal is required to completely dissect IG repertoires. Here, we describe the localization and organization of the rearranging IG heavy (IGH) genes on chromosome 7 of the cynomolgus macaque draft genome. Our annotation comprises 108 functional genes which include 63 variable (IGHV), 38 diversity (IGHD), and 7 joining (IGHJ) genes. For validation, we provide RNA transcript data for most of the IGHV genes and all of the annotated IGHJ genes, as well as proteomic data to validate IGH constant genes. The description and annotation of the rearranging IGH genes for the cynomolgus macaques will significantly facilitate scientific research. This is particularly relevant to dissect the immune response during vaccination or infection with dangerous pathogens such as Ebola, Marburg and other emerging pathogens where non-human primate models play a significant role for countermeasure development.

Keywords

Macaca fascicularis Cynomolgus macaque Variable genes Diversity genes Joining genes V-D-J rearrangement 

Supplementary material

251_2016_921_MOESM1_ESM.docx (35 kb)
ESM 1(DOCX 35 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg (outside the USA) 2016

Authors and Affiliations

  • Guo-Yun Yu
    • 1
  • Suzanne Mate
    • 1
  • Karla Garcia
    • 1
  • Michael D. Ward
    • 2
  • Ernst Brueggemann
    • 2
  • Matthew Hall
    • 1
  • Tara Kenny
    • 2
  • Mariano Sanchez-Lockhart
    • 1
  • Marie-Paule Lefranc
    • 3
  • Gustavo Palacios
    • 1
    • 4
  1. 1.Center for Genome Sciences of the United States Army Medical Research Institute of Infectious Diseases (USAMRIID)Fort DetrickUSA
  2. 2.Molecular Translational Sciences of the United States Army Medical Research Institute of Infectious Diseases (USAMRIID)Fort DetrickUSA
  3. 3.IMGT®, The international ImMunoGeneTics information system®, Laboratoire d’ImmunoGénétique Moléculaire (LIGM)Montpellier UniversityMontpellierFrance
  4. 4.USAMRIID, Center for Genome SciencesFrederickUSA