Immunogenetics

, Volume 56, Issue 12, pp 924–929

Variable domains and a VpreB-like molecule are present in a jawless vertebrate

  • John P. Cannon
  • Robert N. Haire
  • Zeev Pancer
  • M. Gail Mueller
  • Diana Skapura
  • Max D. Cooper
  • Gary W. Litman
Original Paper

DOI: 10.1007/s00251-004-0766-y

Cite this article as:
Cannon, J.P., Haire, R.N., Pancer, Z. et al. Immunogenetics (2005) 56: 924. doi:10.1007/s00251-004-0766-y

Abstract

Immunoglobulins (Igs) and T cell antigen receptors (TCRs) that undergo somatic diversification have not been identified in the two extant orders of jawless vertebrates, which occupy essential positions in terms of understanding the evolution of the emergence of adaptive immunity. Using a single motif-dependent PCR-based approach coupled with a vector that allows selection of cDNAs encoding secretion signal sequences, four different genes encoding Ig V-type domains were identified in the sea lamprey (Petromyzon marinus). One of the predicted proteins encoded by these genes shares structural characteristics with mammalian VpreB molecules, including the absence of a recognizable transmembrane region, a relatively high proportion of charged amino acids in its C-terminal tail and distinctive features of its secretion signal peptide. This is the first indication of a molecule related to the B cell receptor (BCR) complex in a species that diverged prior to the jawed vertebrates in which RAG-mediated adaptive immunity is first encountered.

Keywords

Jawless vertebrateLymphocyte ontogenyV-related sequencePhylogenyImmunoglobulinT cell antigen receptor

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • John P. Cannon
    • 1
    • 2
    • 3
  • Robert N. Haire
    • 3
  • Zeev Pancer
    • 4
  • M. Gail Mueller
    • 3
  • Diana Skapura
    • 3
  • Max D. Cooper
    • 4
  • Gary W. Litman
    • 1
    • 2
    • 3
  1. 1.Department of Molecular GeneticsAll Children’s HospitalSt. PetersburgUSA
  2. 2.H. Lee Moffitt Cancer Center and Research InstituteTampaUSA
  3. 3.Department of Pediatrics, USF/ACH Children’s Research InstituteUniversity of South Florida College of MedicineSt. PetersburgUSA
  4. 4.Howard Hughes Medical InstituteUniversity of Alabama at BirminghamBirminghamUSA