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C60 fullerene affects elastic properties and osmoregulation reactions of human lymphocytes

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Abstract

The influence of aqueous solution of pristine C60 fullerene (C60FAS) on functional activity of lymphocytes from a healthy person was studied for the first time. By means of atomic force microscopy, it was found that C60FAS in a concentration of 0.1 mg/ml increases the stiffness of the lymphocyte membrane by 41 % (p < 0.05) and lowers the functional activity of the plasmalemma surface, thereby constraining the use of its membrane material in physiological reactions using a hypotonic model in vitro. However, a cell retains the ability to regulate its volume and demonstrates relative resistance to hypo-osmotic stress. The resistance of lymphocytes in hypo-osmotic medium is facilitated by activation of the nucleus by C60 fullerene particles, which regulates the implementation of two consistent phases of an increase and decrease of cell volume, thereby retaining cell viability. All these indicate the impact of C60 fullerene on the cellular nucleus.

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References

  • Cherniavskikh SD, Fedorova MZ, Tkhan VV, Kuet DK (2012) The reorganization of the actin cytoskeleton of nuclear erythrocytes and leukocytes in fish, frogs and birds during cell migration. Tsitologiia 54:412–416

    CAS  PubMed  Google Scholar 

  • de Coupade C, Brown AS, Dazin PF, Levine JD, Green PG (2007) β2-Adrenergic receptor-dependent sexual dimorphism for murine leukocyte migration. J Neuroimmunol 186:54–62. doi:10.1016/j.jneuroim.2007.02.010

    Article  PubMed Central  PubMed  Google Scholar 

  • Evstigneev MP, Buchelnikov AS, Voronin DP, Rubin YuV, Belous LF, Prylutskyy YuI, Ritter U (2013) Complexation of C60 fullerene with aromatic drugs. ChemPhysChem 14:568–578. doi:10.1002/cphc.201200938

    Article  CAS  PubMed  Google Scholar 

  • Fedorova MZ, Golovko SI, Chernyavskikh SD (2012) Comparative evaluation of morphofunctional organization of nucleated blood cells of vertebrate animals. J Evol Biochem Phys 48:209–213. doi:10.1134/S0022093012020107

    Article  Google Scholar 

  • Feske S (2007) Calcium signaling in lymphocyte activation and decease. Nat Rev Immunol 7:690–702. doi:10.1038/nri2152

    Article  CAS  PubMed  Google Scholar 

  • Grebowski J, Krokosz A, Puchala M (2013a) Membrane fluidity and activity of membrane ATPases in human erythrocytes under the influence of polyhydroxylated fullerene. Biochim Biophys Acta, Biomembr 1828:241–248. doi:10.1016/j.bbamem.2012.09.008

    Article  CAS  Google Scholar 

  • Grebowski J, Krokosz A, Puchala M (2013b) Fullerenol C60(OH)36 could associate to band 3 protein of human erythrocyte membranes. Biochim Biophys Acta, Biomembr 1828:2007–2014. doi:10.1016/j.bbamem.2013.05.009

    Article  CAS  Google Scholar 

  • Insall RH, Machesky LM (2009) Actin dynamics at the leading edge: from simple machinery to complex networks. Dev Cell 17:310–322. doi:10.1016/j.devcel.2009.08.012

    Article  CAS  PubMed  Google Scholar 

  • Kwon MS, Lee SD, Kim J-A, Colla E, Choi YJ, Suh P-G, Kwon HM (2008) Novel nuclear localization signal regulated by ambient tonicity in vertebrates. J Biol Chem 283:22400–22409. doi:10.1074/jbc.M710550200

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Liu J-H, Cao L, Luo PG, Yang S-T, Lu F, Wang H, Meziani MJ, Haque SA, Liu Y, Lacher S, Sun Y-P (2010) Fullerene-conjugated doxorubicin in cells. ACS Appl Mater Interfaces 2:1384–1389. doi:10.1021/am100037y

    Article  CAS  PubMed  Google Scholar 

  • Mackeyev Y, Raoof M, Cisneros B, Koshkina N, Berger CS, Wilson LJ, Curley SA (2014) Toward paclitaxel–[60]fullerene immunoconjugates as a targeted prodrug against cancer. Nanosyst Phys Chem Math 5:67–75

    Google Scholar 

  • Panchuk RR, Prylutska SV, Chumak VV, Skorokhyd NR, Lehka LV, Evstigneev MP, Prylutskyy YuI, Berger W, Heffeter P, Scharff P, Ritter U, Stoika RS (2015) Application of C60 fullerene-doxorubicin complex for tumor cell treatment in vitro and in vivo. J Biomed Nanotechnol 11:1139–1152. doi:10.1166/jbn.2015.2058

    Article  CAS  Google Scholar 

  • Prylutska S, Grynyuk I, Matyshevska O, Prylutskyy Yu, Evstigneev M, Scharff P, Ritter U (2014) C60 fullerene as synergistic agent in tumor-inhibitory doxorubicin treatment. Drugs R D 14:333–340. doi:10.1007/s40268-014-0074-4

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Prylutskyy YuI, Buchelnikov AS, Voronin DP, Kostjukov VV, Ritter U, Parkinson JA, Evstigneev MP (2013) C60 fullerene aggregation in aqueous solution. Phys Chem Chem Phys 15:9351–9360. doi:10.1039/c3cp50187f

    Article  CAS  PubMed  Google Scholar 

  • Prylutskyy YuI, Evstigneev MP, Pashkova IS, Wyrzykowski D, Woziwodzka A, Golunski G, Piosik J, Cherepanov VV, Ritter U (2014a) Characterization of C60 fullerene complexation with antibiotic doxorubicin. Phys Chem Chem Phys 16:23164–23172. doi:10.1039/C4CP03367A

    Article  CAS  PubMed  Google Scholar 

  • Prylutskyy YuI, Petrenko VI, Ivankov OI, Kyzyma OA, Bulavin LA, Litsis OO, Evstigneev MP, Cherepanov VV, Naumovets AG, Ritter U (2014b) On the origin of C60 fullerene solubility in aqueous solution. Langmuir 30:3967–3970. doi:10.1021/la404976k

    Article  CAS  PubMed  Google Scholar 

  • Ritter U, Prylutskyy YuI, Evstigneev MP, Davidenko NA, Cherepanov VV, Senenko AI, Marchenko OA, Naumovets AG (2015) Structural features of highly stable reproducible C60 fullerene aqueous colloid solution probed by various techniques. Fuller Nanotub Carbon Nanostruct 23:530–534. doi:10.1080/1536383X.2013.870900

    Article  CAS  Google Scholar 

  • Skorkina MYu (2014) Use of plasmalemma reserve by lymphocytes for osmotic stress tests in vitro. Adv Environ Biol 8:46–48

    Google Scholar 

  • Tran-Son-Tay R, Nash G (2007) Mechanical properties of leukocytes and their effects on the circulation. In: Baskurt OG, Hardeman MR, Meiselman HJ, Rampling MW (eds) Handbook of hemorheology and hemodynamics. IOS Press, Amsterdam, pp 127–152

    Google Scholar 

  • Zakharian TY, Seryshev A, Sitharaman B, Gilbert BE, Knight V, Wilson LJ (2005) A fullerene−paclitaxel chemotherapeutic: synthesis, characterization, and study of biological activity in tissue culture. J Am Chem Soc 127:12508–12509. doi:10.1021/ja0546525

    Article  CAS  PubMed  Google Scholar 

  • Zhang X, Zhang Y, Zheng Y, Wang B (2013) Mechanical characteristics of human red blood cell membrane change due to C60 nanoparticle infiltration. Phys Chem Chem Phys 15:2473–2481. doi:10.1039/C2CP42850D

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We thank the Russian Science Fund (project No. 14-14-00328) for financial support and also appreciate the participation of anonymous donors.

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Authors and Affiliations

  1. Belgorod State University, 85 Pobedy Str., 308015, Belgorod, Russia

    Marina Yu. Skorkina, Evgenia A. Sladkova, Elena A. Shamray, Maxim P. Evstigneev & Anatoly S. Buchelnikov

  2. Clinical-diagnostic Laboratory, Hematological Department, St. Ioasaf Regional Hospital, 8/9 Nekrasova Str., 308007, Belgorod, Russia

    Olga V. Cherkashina

  3. Sevastopol State University, 33 Universitetskaya Str., 299053, Sevastopol, Crimea

    Maxim P. Evstigneev

  4. Taras Shevchenko National University of Kyiv, 64 Volodymyrska Str., Kyiv, 01601, Ukraine

    Yuriy I. Prylutskyy

  5. Ilmenau University of Technology, Institute for Chemistry and Biotechnology, 25 Weimarer Str., 98693, Ilmenau, Germany

    Uwe Ritter

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  1. Marina Yu. Skorkina
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  2. Evgenia A. Sladkova
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  3. Elena A. Shamray
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  4. Olga V. Cherkashina
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  5. Maxim P. Evstigneev
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  6. Anatoly S. Buchelnikov
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  7. Yuriy I. Prylutskyy
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  8. Uwe Ritter
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Correspondence to Marina Yu. Skorkina.

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Skorkina, M.Y., Sladkova, E.A., Shamray, E.A. et al. C60 fullerene affects elastic properties and osmoregulation reactions of human lymphocytes. Eur Biophys J 44, 493–498 (2015). https://doi.org/10.1007/s00249-015-1050-9

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  • DOI: https://doi.org/10.1007/s00249-015-1050-9

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