Original Paper

European Biophysics Journal

, Volume 38, Issue 6, pp 807-812

Structured illumination microscopy of a living cell

  • Liisa M. HirvonenAffiliated withRandall Division of Cell and Molecular Biophysics, King’s College London
  • , Kai WickerAffiliated withRandall Division of Cell and Molecular Biophysics, King’s College London
  • , Ondrej MandulaAffiliated withFaculty of Mathematics and Physics, Charles University
  • , Rainer HeintzmannAffiliated withRandall Division of Cell and Molecular Biophysics, King’s College London Email author 

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Abstract

Due to diffraction, the resolution of imaging emitted light in a fluorescence microscope is limited to about 200 nm in the lateral direction. Resolution improvement by a factor of two can be achieved using structured illumination, where a fine grating is projected onto the sample, and the final image is reconstructed from a set of images taken at different grating positions. Here we demonstrate that with the help of a spatial light modulator, this technique can be used for imaging slowly moving structures in living cells.

Keywords

Structured illumination microscopy Live-cell imaging Resolution improvement