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A simulation study on the Ca2+-independent but voltage-dependent exocytosis and endocytosis in dorsal root ganglion neurons

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Abstract

In patch-clamped somata of dorsal root ganglion (DRG) neurons, two types of secretion have been proposed: Ca2+-dependent secretion and Ca2+-independent but voltage-dependent secretion (CIVDS). The Ca2+-induced and the depolarization-induced membrane capacitance (Cm) increases contribute 80 and 20% to the total Cm increase, respectively (Zhang and Zhou in Nat Neurosci 5:425, 2002). In order to explore the mechanism of the voltage-dependent Cm change (ΔCm), we constructed a model with sequential states. The simulation with this model closely approximates all the experimental data. The model predicts that the majority of fusion events (approximately 80%) are so-called “kiss-and-run” events, which account for the fast recovery or the rapid retrieval feature of the signals. The remaining 20% are attributed to full fusion events, which account for a slow retrieval feature. On the basis of the model, one mechanism of the activity-dependent endocytosis has revealed a differential distribution of vesicles between the kiss-and-run and full fusion states at different stimulation frequencies. The quantitative model presented in this study may help us to understand the mechanism of the CIVDS and the tightly coupled endocytosis found in mammalian DRG neurons.

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Acknowledgements

We thank Chris Lingle, Steven Mennerick and Iain Bruce for comments on the manuscript. This work was supported by grants from the National Science Foundation of China (30025023, 3000062 30130230, 30328013 and 30330210), the Major State Basic Research Program of the P.R. China (G1999054000, 2001CCA04100 and G2000077800), the Li Foundation and the Sinogerman Scientific Center.

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Authors and Affiliations

  1. Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China

    Hua Yang, Tao Xu & Jiu Ping Ding

  2. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Shanghai, 200031, China

    Tao Xu

  3. Institute of Molecular Medicine, Peking University, Beijing, 100871, China

    Hui Zheng, Wei Xiong & Zhuan Zhou

  4. Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China

    Chen Zhang, Hui Zheng, Wei Xiong & Zhuan Zhou

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  1. Hua Yang
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  2. Chen Zhang
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  3. Hui Zheng
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  4. Wei Xiong
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  5. Zhuan Zhou
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Correspondence to Jiu Ping Ding.

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Yang, H., Zhang, C., Zheng, H. et al. A simulation study on the Ca2+-independent but voltage-dependent exocytosis and endocytosis in dorsal root ganglion neurons. Eur Biophys J 34, 1007–1016 (2005). https://doi.org/10.1007/s00249-005-0486-8

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  • DOI: https://doi.org/10.1007/s00249-005-0486-8

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