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Ericoid Roots and Mycospheres Govern Plant-Specific Bacterial Communities in Boreal Forest Humus

  • Soil Microbiology
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Abstract

In this study, the bacterial populations of roots and mycospheres of the boreal pine forest ericoid plants, heather (Calluna vulgaris), bilberry (Vaccinium myrtillus), and lingonberry (Vaccinium vitis-idaea), were studied by qPCR and next-generation sequencing (NGS). All bacterial communities of mycosphere soils differed from soils uncolonized by mycorrhizal mycelia. Colonization by mycorrhizal hyphae increased the total number of bacterial 16S ribosomal DNA (rDNA) gene copies in the humus but decreased the number of different bacterial operational taxonomic units (OTUs). Nevertheless, ericoid roots and mycospheres supported numerous OTUs not present in uncolonized humus. Bacterial communities in bilberry mycospheres were surprisingly similar to those in pine mycospheres but not to bacterial communities in heather and lingonberry mycospheres. In contrast, bacterial communities of ericoid roots were more similar to each other than to those of pine roots. In all sample types, the relative abundances of bacterial sequences belonging to Alphaproteobacteria and Acidobacteria were higher than the sequences belonging to other classes. Soil samples contained more Actinobacteria, Deltaproteobacteria, Opitutae, and Planctomycetia, whereas Armatimonadia, Betaproteobacteria, Gammaproteobacteria, and Sphingobacteriia were more common to roots. All mycosphere soils and roots harbored bacteria unique to that particular habitat. Our study suggests that the habitation by ericoid plants increases the overall bacterial diversity of boreal forest soils.

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Acknowledgements

We gratefully acknowledge Academy of Finland (grant numbers 131819, 263858, 292699), the research funding for Jiangsu Specially Appointed Professor (project 165010015), and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). Sincere thanks to Minna Santalahti for the upkeep of the plants as well as help in the sampling.

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Authors and Affiliations

  1. Department of Food and Environmental Sciences, Division of Microbiology and Biotechnology, University of Helsinki, P.O. Box 56, 00014, Helsinki, FIN, Finland

    Sari Timonen, Hanna Sinkko, Hui Sun, Outi-Maaria Sietiö, Johanna M Rinta-Kanto, Heikki Kiheri & Jussi Heinonsalo

  2. Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, China

    Hui Sun

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  6. Heikki Kiheri
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  7. Jussi Heinonsalo
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Corresponding author

Correspondence to Sari Timonen.

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Funding

This study was funded by Academy of Finland (grant numbers 131819, 263858, 292699), the research funding for Jiangsu Specially Appointed Professor (project 165010015), and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Conflict of Interest

The authors declare that they have no conflict of interest.

Additional information

The nucleotide sequence data reported are available in the NCBI database under the study number SRP0928835.

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Timonen, S., Sinkko, H., Sun, H. et al. Ericoid Roots and Mycospheres Govern Plant-Specific Bacterial Communities in Boreal Forest Humus. Microb Ecol 73, 939–953 (2017). https://doi.org/10.1007/s00248-016-0922-6

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