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Microbial Community Dynamics and Response to Plant Growth-Promoting Microorganisms in the Rhizosphere of Four Common Food Crops Cultivated in Hydroponics

  • Plant Microbe Interactions
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Abstract

Plant growth promoting microorganisms (PGPMs) of the plant root zone microbiome have received limited attention in hydroponic cultivation systems. In the framework of a project aimed at the development of a biological life support system for manned missions in space, we investigated the effects of PGPMs on four common food crops (durum and bread wheat, potato and soybean) cultivated in recirculating hydroponic systems for a whole life cycle. Each crop was inoculated with a commercial PGPM mixture and the composition of the microbial communities associated with their root rhizosphere, rhizoplane/endosphere and with the recirculating nutrient solution was characterised through 16S- and ITS-targeted Illumina MiSeq sequencing. PGPM addition was shown to induce changes in the composition of these communities, though these changes varied both between crops and over time. Microbial communities of PGPM-treated plants were shown to be more stable over time. Though additional development is required, this study highlights the potential benefits that PGPMs may confer to plants grown in hydroponic systems, particularly when cultivated in extreme environments such as space.

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Acknowledgments

This research was supported by the MELiSSA project from the European Space Agency under contract no. 400010819/13/NL/JC.

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Authors and Affiliations

  1. In Vitro Biology and Horticulture, Department of Plant Production, Faculty of Bioscience Engineering, Ghent University, 653 Coupure Links, 9000, Ghent, Belgium

    C. Sheridan, P Depuydt, M. De Ro, C. Petit, E. Van Gysegem, P. Delaere & D. Geelen

  2. Controlled Environment Systems Research Facility, School of Environmental Sciences, University of Guelph, Guelph, ON, NIG 2W1, Canada

    M. Dixon & M. Stasiak

  3. Institute of Agricultural Sciences, ETH Zurich, Zurich, Switzerland

    S. B. Aciksöz & E. Frossard

  4. Division of Plant Biology and Crop Science, Department of Agricultural Sciences, University of Naples Federico II Naples, Via Università, 100 80055, Portici, Naples, Italy

    R. Paradiso & S. De Pascale

  5. Department of Mathematical Modelling, Statistics and Bioinformatics, Ghent University, Ghent, Belgium

    T. De Meyer

  6. Department of Food Quality and Food Safety, Ghent University, Coupure links 653, B-9000, Ghent, Belgium

    B. Sas

  7. Division of Microbiology, Department of Agricultural Sciences, University of Naples Federico II Naples, Via Università, 100 80055, Portici, Naples, Italy

    V. Ventorino

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  1. C. Sheridan
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Fig. S1

Rarefaction curves (±SD) representing the number of OTUs discovered in control (red) and inoculated (blue) durum wheat, and control (yellow) and inoculated (green) soybean. (GIF 189 kb)

High resolution image (EPS 6224 kb)

Fig. S2

Rarefaction curves (±SD) representing the number of OTUs discovered in control (red) and inoculated (blue) bread wheat, control (green) and inoculated (purple) soybean, and in the Myco Madness PGPM mix (yellow). (GIF 176 kb)

High resolution image (EPS 4935 kb)

Fig. S3

PCoA analysis showing Bray-Curtis similarity values of evenly sub-sampled bread wheat- (A), durum wheat- (B), potato- (C) and soybean- associated (D) communities. NS_treat= nutrient solution samples treated with Myco Madness, NS_con= control nutrient solution samples, En_Con= control endosphere/rhizoplane samples, En_Treat= endosphere/rhizoplane samples from Myco Madness-treated plants, Ex_Con= control rhizosphere samples, Ex_Treat= rhizosphere samples from Myco Madness-treated plants. (GIF 52 kb)

High resolution image (EPS 545 kb)

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Sheridan, C., Depuydt, P., De Ro, M. et al. Microbial Community Dynamics and Response to Plant Growth-Promoting Microorganisms in the Rhizosphere of Four Common Food Crops Cultivated in Hydroponics. Microb Ecol 73, 378–393 (2017). https://doi.org/10.1007/s00248-016-0855-0

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